JOVE JOURNAL

Biochemistry

Showing 205 - 216 in 731 videos.

Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions

Robert P. Sparks*¹, William Lawless*², Andres S. Arango*^1,3,4, Emad Tajkhorshid^3,4, Rutilio A. Fratti³

¹Department of Biochemistry,University of Illinois at Urbana-Champaign, ²Department of Chemistry,University of South Florida, ³Center for Biophysics and Quantitative Biology,University of Illinois at Urbana-Champaign, ⁴Beckman Institute for Advanced Science and Technology,University of Illinois at Urbana-Champaign

Journal (Biochemistry)

The Extraction of Liver Glycogen Molecules for Glycogen Structure Determination

Ziyi Wang², Qinghua Liu⁴, Liang Wang⁵, Robert G. Gilbert^2,6, Mitchell A. Sullivan

¹School of Chemistry and Molecular Biosciences,The University of Queensland, ²Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation,The University of Queensland, ³Jiangsu Provincial Key Laboratory of New Drug Research and Clinical Pharmacy, School of Pharmacy,Xuzhou Medical University, ⁴Department of Pharmaceutical Analysis, School of Pharmacy,Xuzhou Medical University, ⁵Department of Bioinformatics, School of Medical Informatics and Engineering,Xuzhou Medical University, ⁶Joint International Research Laboratory of Agriculture and Agri-Product Safety, College of Agriculture,Yangzhou University, ⁷Glycation and Diabetes Group,Mater Research Institute-The University of Queensland, Translational Research Institute

Journal (Biochemistry)

Quantification of Subcellular Glycogen Distribution in Skeletal Muscle Fibers using Transmission Electron Microscopy

Rasmus Jensen, Niels Ørtenblad, Cristiano di Benedetto, Klaus Qvortrup, Joachim Nielsen

¹Research center for applied health science,University College South Denmark, ²Department of Sports Science and Clinical Biomechanics,University of Southern Denmark, ³Department of Biomedical Sciences, Core Facility for Integrated Microscopy,University of Copenhagen

Journal (Biochemistry)

Manual Blot-and-Plunge Freezing of Biological Specimens for Single-Particle Cryogenic Electron Microscopy

Hoang P. M. Nguyen*¹, Kelly L. McGuire*¹, Brian D. Cook*¹, Mark A. Herzik, Jr.

¹Department of Chemistry and Biochemistry,University of California, San Diego

Journal (Biochemistry)

Motility of Single Molecules and Clusters of Bi-Directional Kinesin-5 Cin8 Purified from S. cerevisiae Cells

Himanshu Pandey, Tatiana Zvagelsky, Mary Popov, Mayan Sadan, Neta Yanir, Alina Goldstein-Levitin, Nurit Siegler, Shira Hershfinkel, Yahel Abraham, Roy Avraham, Levi A. Gheber, Larisa Gheber

¹Department of Chemistry,Ben-Gurion University of the Negev, Israel, ²Department of Biotechnology Engineering,Ben-Gurion University of the Negev, Israel

Journal (Biochemistry)

A Robust Single-Particle Cryo-Electron Microscopy (cryo-EM) Processing Workflow with cryoSPARC, RELION, and Scipion

Megan C. DiIorio, Arkadiusz W. Kulczyk²

¹Institute for Quantitative Biomedicine,Rutgers University, ²Department of Biochemistry & Microbiology,Rutgers University

Journal (Biochemistry)

Transverse Sectioning of Mature Rice (Oryza sativa L.) Kernels for Scanning Electron Microscopy Imaging Using Pipette Tips as Immobilization Support

Jordan Demone, Kiah A. Barton², Illimar Altosaar²

¹Department of Biochemistry, Microbiology, and Immunology,University of Ottawa, ²Proteins Easy Corp

Journal (Biochemistry)

Ready-To-Use qPCR for Detection of DNA from Trypanosoma cruzi or Other Pathogenic Organisms

Alexandre Dias Tavares Costa^2,3, Steffanie Skau Amadei³, Amanda Bertão-Santos², Tuany Rodrigues³

¹Laboratório de Ciências e Tecnologias Aplicadas em Saúde (LaCTAS), Instituto Carlos Chagas (ICC),Fundação Oswaldo Cruz (Fiocruz), ²Pós-graduação em Engenharia de Bioprocessos e Biotecnologia,Universidade Federal do Paraná (UFPR), ³Pós-graduação em Biociências e Biotecnologia, Instituto Carlos Chagas (ICC),Fundação Oswaldo Cruz (Fiocruz)

Journal (Biochemistry)

Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B

Zekiye Ceren Arituluk², Bishnu Adhikari, Urmila Maitra, Caroline Goodman, Lukasz M. Ciesla

¹Department of Biological Sciences,The University of Alabama, ²Department of Pharmaceutical Botany, Faculty of Pharmacy,Hacettepe University

Journal (Biochemistry)

Synthesis of Masarimycin, a Small Molecule Inhibitor of Gram-Positive Bacterial Growth

Mika Gallati, Bryant Point, Christopher W. Reid²

¹Department of Science and Technology,Bryant University, ²Center for Health and Behavioral Sciences,Bryant University

Journal (Biochemistry)

Measurement of Protein Import Capacity of Skeletal Muscle Mitochondria

Ashley N. Oliveira, Brandon J. Richards, David A. Hood

¹Muscle Health Research Center, School of Kinesiology and Health Science,York University

Journal (Biochemistry)

Quantifying Bacterial Surface Swarming Motility on Inducer Gradient Plates

Siyu Guo, Zhao Liu, Yongshuai Yang, Jun Chen, Chun Loong Ho

¹Department of Biomedical Engineering,Southern University of Science and Technology

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