Jove Lab Chem
Basic Organic Chemistry Techniques — Lab Prep
Source: Lara Al Hariri at the University of Massachusetts Amherst, MA, USA
- Preparation of the Laboratory
Here, we show the laboratory preparation for 10 students working in pairs, with some excess. Please adjust quantities as needed.
-
To set up for this lab experiment, wear the appropriate personal protective equipment, including a lab coat, chemical splash goggles, and gloves.
-
Ensure that there are labeled waste containers for non-halogenated organic waste, hydrochloric acid waste, aqueous waste, and glass waste.
-
Place a container of sodium benzoate and a small waste container for excess sodium benzoate by the balances.
-
Ensure that the balances have enough weighing boats, spatulas, and lab wipes for the class.
-
In a fume hood, place 150 mL of tetrahydrofuran in a 250-mL glass bottle. Cap the bottle and label it ‘tetrahydrofuran’.
-
Carefully place 35 mL of 3 M hydrochloric acid in three 50-mL glass bottles. Cap the bottles and label them with the solution's name and concentration.
-
Place these bottles of THF and HCl in a central hood and put away the stock bottles.
-
Set out the following glassware and equipment at each student lab station (we suggest that students work in pairs):
2 Lab stands 1 Stirring hotplate 1 Lab jack 1 Small thermometer clamp 3 Medium 3-prong clamp 1 Condenser 2 Rubber tubing 2 Vacuum tubing 3 50-mL beaker 2 250-mL beaker 1 600-mL beaker 1 5-mL graduated cylinder 1 10-mL graduated cylinder 1 50-mL graduated cylinder 1 250-mL filter flask 1 50-mL round-bottom flask with cork holder 1 Büchner funnel with rubber adaptor 2 Pasteur pipette bulbs 1 Stir bar 1 Glass stirring rod 1 Roll of lab tape with pen 1 Thermometer -
Fill plastic wash bottles with deionized water and distribute them around the lab. Set out a tube of vacuum grease and boxes of circular filter papers, laboratory wipes, and Pasteur pipettes in a central location.
-