All Protocols and Video Articles for scientific research | JoVE

Showing 25 - 36 of 108 videos for "EcoRI"

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Journal (Genetics)

Genetic Variant Detection in the CALR gene using High Resolution Melting Analysis

Tadej Pajič², Tanja Belčič Mikič, Helena Podgornik³, Jurka Klun, Sandra Šućurović, Samo Zver, Matjaz Sever

¹Department of Hematology,University Medical Centre Ljubljana, ²Clinical Institute for Genomic Medicine,University Medical Centre Ljubljana, ³Faculty of Pharmacy,University of Ljubljana

Journal (Genetics)

CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis

Andi M. Wilson², Brenda D. Wingfield²

¹Department of Biochemistry, Genetics & Microbiology,University of Pretoria, ²Forestry & Agricultural Biotechnology Institute (FABI),University of Pretoria

Journal (Biology)

Parallel High Throughput Single Molecule Kinetic Assay for Site-Specific DNA Cleavage

Emily K. Matozel, Nathaniel Dale, Allen C. Price

¹Department of Biology,Emmanuel College, ²Department of Chemistry and Physics,Emmanuel College

Journal (Immunology and Infection)

Rapid, Seamless Generation of Recombinant Poxviruses using Host Range and Visual Selection

Sameera Vipat*¹, Greg Brennan*¹, Chorong Park, Sherry L. Haller, Stefan Rothenburg

¹Department of Medial Microbiology and Immunology,School of Medicine, University of California Davis, ²Department of Microbiology and Immunology,University of Texas Medical Branch at Galveston

Journal (Chemistry)

Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro

Andreas Schreiber*^1,2, Lara G. Stühn*^1,2, Süreyya E. Geissinger², Matthias C. Huber², Stefan M. Schiller^2,3,4,5,6

¹Center for Biological Systems Analysis,University of Freiburg, ²Faculty of Biology,University of Freiburg, ³Freiburg Institute for Advanced Studies (FRIAS),University of Freiburg, ⁴BIOSS Centre for Biological Signalling Studies,University of Freiburg, ⁵IMTEK Department of Microsystems Engineering,University of Freiburg, ⁶Cluster of Excellence livMatS at FIT - Freiburg Center for Interactive Materials and Bioinspired Technologies,University of Freiburg

Journal (Genetics)

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

Krzysztof J. Skowronek, Matthias Bochtler

¹International Institute of Molecular and Cell Biology, Warsaw (IIMCB)

Journal (Neuroscience)

Assessing Early Stage Open-Angle Glaucoma in Patients by Isolated-Check Visual Evoked Potential

Xiang Fan², Lingling Wu², Aihua Ding²

¹Department of Ophthalmology,Peking University Third Hospital, ²Beijing Key Laboratory of Restoration of Damaged Ocular Nerve,Peking University Third Hospital

Journal (Immunology and Infection)

Generation of In-Frame Gene Deletion Mutants in Pseudomonas aeruginosa and Testing for Virulence Attenuation in a Simple Mouse Model of Infection

Meagan E. Valentine, Brandon D. Kirby, Hongwei D. Yu²

¹Progenesis Technologies, LLC, ²Department of Biomedical Sciences, Pediatrics,Joan C. Edwards School of Medicine at Marshall University

Journal (Cancer Research)

Isolation of Exosome-Enriched Extracellular Vesicles Carrying Granulocyte-Macrophage Colony-Stimulating Factor from Embryonic Stem Cells

Shuhan Meng², Aaron G. Whitt^2,3, Allison Tu, John W. Eaton^2,3, Chi Li^2,3, Kavitha Yaddanapudi^5,6

¹Department of Pharmacology and Toxicology,University of Louisville, ²Experimental Therapeutics Program, Brown Cancer Center,University of Louisville, ³Department of Medicine,University of Louisville, ⁴Department of Surgery,University of Louisville, ⁵Immuno-Oncology Program, Brown Cancer Center,University of Louisville, ⁶Department of Microbiology and Immunology,University of Louisville

Journal (Genetics)

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

Fabian L. Cardenas-Diaz*¹, Jean Ann Maguire*¹, Paul Gadue^2,3, Deborah L. French^2,3

¹Center for Cellular and Molecular Therapeutics,The Children's Hospital of Philadelphia, ²Department of Pathology and Laboratory Medicine,The Children's Hospital of Philadelphia, ³Department of Pathology and Laboratory Medicine,University of Pennsylvania

Journal (Biochemistry)

Monitoring GPCR-β-arrestin1/2 Interactions in Real Time Living Systems to Accelerate Drug Discovery

Arfaxad Reyes-Alcaraz, Yoo-Na Lee, Seongsik Yun, Jong-Ik Hwang, Jae Young Seong

¹Graduate School of Medicine,Korea University

Journal (Genetics)

Modulation of Tau Subcellular Localization as a Tool to Investigate the Expression of Disease-related Genes

Giacomo Siano, Maria Claudia Caiazza, Martina Varisco, Mariantonietta Calvello, Valentina Quercioli, Antonino Cattaneo, Cristina Di Primio

¹Laboratory of Biology, BIO@SNS,Scuola Normale Superiore

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