All Protocols and Video Articles for scientific research | JoVE

Showing 1 - 12 of 486 videos for "Centrin-EGFP"

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Journal (Neuroscience)

Ex Utero Electroporation and Organotypic Slice Cultures of Embryonic Mouse Brains for Live-Imaging of Migrating GABAergic Interneurons

Lara Eid², Mathieu Lachance, Gilles Hickson³, Elsa Rossignol^2,4

¹Centre de recherche du CHU Sainte-Justine, ²Department of Neuroscience,Université de Montréal, ³Department of pathology and cellular biology,Université de Montréal, ⁴Department of Pediatrics,Université de Montréal

Journal (Biology)

Cell-Free Protein Synthesis in Deployable Hydrogels

Siji Kavil*¹, Alex Laverick*¹, Colette J. Whitfield, Alice M. Banks, Thomas P. Howard

¹School of Natural and Environmental Sciences,Newcastle University, ²Department of Life Sciences,Imperial College London

Journal (Biology)

Assessment of EGFP-Positive Fiber Electrical Activity, Cysteine Staining, and Signal Acquisition

Hugo Bibollet, Daniel F. Bennett, Martin F. Schneider, Erick O. Hernández-Ochoa

¹Department of Biochemistry and Molecular Biology,University of Maryland School of Medicine

Journal (Biology)

Gold Nanoparticle Synthesis for Mammalian Cells Using Sapphire Discs

¹National Institute of Biological Sciences, Beijing, ²Tsinghua Institute of Multidisciplinary Biomedical Research,Tsinghua University

Journal (Biology)

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Methods Collections

Transgene Expression in Cultured Cells Using Unpurified Recombinant Adeno-Associated Viral Vectors

Brian Benyamini, Meagan N. Esbin², Oscar Whitney, Nike Walther, Anna C. Maurer

¹Department of Molecular and Cell Biology,University of California, Berkeley, ²Biophysics Graduate Group,University of California, Berkeley

Journal (Neuroscience)

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Methods Collections

Targeted Knockdown of Genes in the Choroid Plexus

Yuwen Yang*¹, Chenxing Qi*¹, Lanxin Hu, Cheng Zheng, Xuhang Li, Wu Zheng, Yiyun Weng, Haiyan Lin⁴

¹State Key Laboratory of Ophthalmology, Optometry and Visual Science, Eye Hospital,Wenzhou Medical University, ²Department of Neurology,the First Affiliated Hospital of Wenzhou Medical University, ³Rehabilitation Medicine Center,The Second Affiliated Hospital of Wenzhou Medical University, ⁴Integrative & Optimized Medicine Research Center, China-USA Institute for Acupuncture and Rehabilitation,Wenzhou Medical University

Journal (Developmental Biology)

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Methods Collections

Live-Cell Imaging of Drosophila melanogaster Third Instar Larval Brains

Roberto Carlos Segura, Clemens Cabernard

¹Department of Biology,University of Washington

Journal (Biology)

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Methods Collections

Methods for Embedding Cell-Free Protein Synthesis Reactions in Macro-Scale Hydrogels

Siji Kavil*¹, Alex Laverick*¹, Colette J. Whitfield, Alice M. Banks, Thomas P. Howard

¹School of Natural and Environmental Sciences,Newcastle University, ²Department of Life Sciences,Imperial College London

Journal (Biology)

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Methods Collections

A Validatable Droplet Digital Polymerase Chain Reaction Assay for the Detection of Adeno-Associated Viral Vectors in Bioshedding Studies of Tears

Bryanna Longacre, Amanda Souaysene, Carrie A. Vyhlidal, Matthew R. Pennington

¹Cell and Gene Therapy,KCAS Bioanalytical and Biomarker Services

Journal (Neuroscience)

Transpupillary-Guided Trans-Scleral Transplantation of Subretinal Grafts in a Retinal Degeneration Mouse Model

Ying V. Liu, Kang V. Li, Zhuolin Li, Yuchen Lu, Minda M. McNally, Edward P. Esposito, Kanza Aziz, Mandeep S. Singh²

¹Wilmer Eye Institute,Johns Hopkins University School of Medicine, ²Department of Genetic Medicine,Johns Hopkins University School of Medicine

Journal (Biology)

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Methods Collections

Imaging ATG9A, a Multi-Spanning Membrane Protein

Alexander R. van Vliet*^1,2, Stefano De Tito*¹, Eugenia Almacellas*¹, Sharon A. Tooze

¹Molecular Cell Biology of Autophagy,The Francis Crick Institute, ²MRC Laboratory of Molecular Biology

Journal (Biology)

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Methods Collections

Generation and Manipulation of Rat Intestinal Organoids

Eleanor Zagoren*¹, Anderson K. Santos*^1,2, Nadia A. Ameen⁴, Kaelyn Sumigray^5,6

¹Department of Genetics,Yale School of Medicine, ²Department of Pediatrics,Yale School of Medicine, ³Department of Pediatrics/Gastroenterology and Hepatology,Yale School of Medicine, ⁴Department of Cellular and Molecular Physiology,Yale School of Medicine, ⁵Yale Stem Cell Center,Yale School of Medicine, ⁶Yale Cancer Center,Yale School of Medicine

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