脊髓电
Summary
一个孤立的新生小鼠脊髓电生理研究的示范。
Abstract
新生小鼠脊髓是一个模型，为研究神经设计电路和运动运动的发展。我们表明脊髓的解剖和准备录音浴人工脑脊液的运动研究中使用的液体。脊髓腹侧神经根的解剖，可以连接到记录电极记录的腰椎脊髓内中央格局产生电路的电生理信号。
Protocol
1。准备人工脑脊液（ACSF）1。首先，我们准备了学联的10倍的股票，没有一个2L的镁或钙。在毫上市的试剂。目录号是指西格玛/爱秩序。 2升10X学联（无镁或钙） 试剂 mM的 g/2L 目录 氯化钾 40 5.96 P – 9333 氯化钠 1280 14…
Discussion
隔离新生儿脊髓提供了一个研究神经系统development1，2的的易处理的方法。在新生儿啮齿类动物的腰椎脊髓中枢模式产生电路，可以产生神经递质的存在虚构的运动。这个虚构的运动节奏活动增加，阵阵，在0.2至0.5赫兹生产。这些爆发是有组织的生产线和屈，伸交替（同侧L2相到L5）的长度沿左，右交替。在小鼠体内的一些基因突变已被证明有异常行为3-5。了解如何在发展和遗传扰动改变的脊髓神?…
Disclosures
The authors have nothing to disclose.
Acknowledgements
塞缪尔百福是在索尔克生物研究所的基因表达实验室教授和霍华德休斯医学研究所研究员。这项工作是由克里斯托弗和Dana里夫基金会的支持。乔Belcovson，肯特Schnoeker和迈克沙利文在索尔克研究所的多媒体资源提供援助，摄影和剪辑。
Materials
| Material Name | Type | Company | Catalogue Number | Comment |
|---|---|---|---|---|
| KCl | P-9333 | |||
| NaCl | S-7653 | |||
| NaHCO3 | S-6297 | |||
| NaH2PO4 | S-9638 | |||
| Glucose | D-9434 | |||
| CaCl2 | C-5080 | |||
| MgSO4 | M-5921 | |||
| Large Scissors | Fine Science Tools | 14070-12 | ||
| Forceps | Fine Science Tools | 11050-10 | ||
| Fine Scissors | Fine Science Tools | 15000-10 | ||
| Insect pins | Fine Science Tools | 26002-10 | ||
| Sylgard 184 (Dow-Corning) | ||||
| 1L volumetric flask | ||||
| 100mL volumetric flask |
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Cite This Article
Meyer, A., Gallarda, B. W., Pfaff, S., Alaynick, W. Spinal Cord Electrophysiology. J. Vis. Exp. (35), e1660, doi:10.3791/1660 (2010).