कम तनाव का चूहे में Lashley III भूलभुलैया का उपयोग करना सीखना

Summary

Lashley क्ष्क्ष्क्ष् भूलभुलैया एक कार्य मार्ग सीखने कि aversive उत्तेजनाओं या दृश्य cues पर भरोसा नहीं करता है. यह इस तरह उम्र बढ़ने चूहों में विशेष रूप से या अन्यथा तनाव एक विचार है, जहां सीखने और स्मृति के मूल्यांकन के लिए एक अत्यंत आकर्षक विकल्प है.

Abstract

Many behavior tests designed to assess learning and memory in rodents, particularly mice, rely on visual cues, food and/or water deprivation, or other aversive stimuli to motivate task acquisition. As animals age, sensory modalities deteriorate. For example, many strains of mice develop hearing deficits or cataracts. Changes in the sensory systems required to guide mice during task acquisition present potential confounds in interpreting learning changes in aging animals. Moreover, the use of aversive stimuli to motivate animals to learn tasks is potentially confounding when comparing mice with differential sensitivities to stress. To minimize these types of confounding effects, we have implemented a modified version of the Lashley III maze. This maze relies on route learning, whereby mice learn to navigate a maze via repeated exposure under low stress conditions, e.g. dark phase, no food/water deprivation, until they navigate a path from the start location to a pseudo-home cage with 0 or 1 error(s) on two consecutive trials. We classify this as a low-stress behavior test because it does not rely on aversive stimuli to encourage exploration of the maze and learning of the task. The apparatus consists of a modular start box, a 4-arm maze body, and a goal box. At the end of the goal box is a pseudo-home cage that contains bedding similar to that found in the animal’s home cage and is specific to each animal for the duration of maze testing. It has been demonstrated previously that this pseudo-home cage provides sufficient reward to motivate mice to learn to navigate the maze¹. Here, we present the visualization of the Lashley III maze procedure in the context of evaluating age-related differences in learning and memory in mice along with a comparison of learning behavior in two different background strains of mice. We hope that other investigators interested in evaluating the effects of aging or stress vulnerability in mice will consider this maze an attractive alternative to behavioral tests that involve more stressful learning tasks and/or visual cues.

Protocol

1. तैयारी: Lashley क्ष्क्ष्क्ष् भूलभुलैया Plexiglas का निर्माण किया है और चित्र 1 में दिखाया गया है है. प्रारंभ बॉक्स में, भूलभुलैया, हथियार, और लक्ष्य बॉक्स सफाई और भूलभुलैया के modifiability के लिए अनुमति देने के लिए म?…

Discussion

सबसे अधिक इस्तेमाल किया कृंतक व्यवहार करने के लिए सीखने और स्मृति का आकलन परीक्षण मॉरिस पानी भूलभुलैया और ^डर 2 कंडीशनिंग के वेरिएंट हैं . इन परीक्षणों सीखने और aversive उत्तेजनाओं (जैसे, पानी, footshock) के लिए ?…

Materials

Material Name	Type	Company	Catalogue Number	Comment
Lashley III maze		PSU Physics Machine Shop	N/A	Maze must be custom-built, not commercially available