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Cancer Research
Mitochondrial Isolation for Transmitochondrial Cybrid Generation (Video) | JoVE
Published: March 17, 2023
doi:
10.3791/200013
Ruth Soler-Agesta
,
Joaquín Marco-Brualla
,
Patricio Fernández-Silva
2
,
Pilar Mozas
3
,
Alberto Anel
,
Raquel Moreno Loshuertos
2
1
Department of Biochemistry
,
University of Zaragoza
,
2
Institute for Biocomputation and Physics of Complex Systems
,
University of Zaragoza
,
3
Sequencing and Functional Genomics Unit
,
University of Zaragoza-IACS
Tags
Mitochondrial Isolation
Transmitochondrial Cybrid Generation
Centrifugation
Homogenization
Hypotonic Buffer
Mitochondrial Protein Quantification
Bradford Assay
Nuclear Contamination Assessment
Quantitative PCR
check_url/200013?article_type=t
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DOI
Cite This Article
Mitochondrial Isolation for Transmitochondrial Cybrid Generation.
J. Vis. Exp.
(Pending Publication), e200013, doi:10.3791/200013 (2023).
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