JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
JoVE Journal > Cancer Research
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Please note that all translations are automatically generated.
Click here for the English version.
Cancer Research
종양 오가노이드 및 일차 섬유아세포를 확립하기 위한 새로운 일차 종양 조직 처리
Published: May 26, 2023
doi:
10.3791/200204
Jesús Frutos Díaz-Alejo*
1,2,3,4
,
Simon April-Monn*
5
,
Marina Cihova
,
Verona Buocikova
,
Jorge Villalón López
3
,
Maria Urbanova
,
Carmen G. Lechuga
,
Miroslav Tomas
8
,
Peter Dubovan
8
,
Bárbara Luna Sánchez
,
Sonia Camaño Páez
,
Alfonso Sanjuanbenito
9
,
Eduardo Lobo
,
Estefanía Romio de la Heras
,
Carmen Guerra
7
,
Carolina de la Pinta
,
Emma Barreto Melian
2
,
Mercedes Rodríguez Garrote
2
,
Alfredo Carrato
2,4
,
Laura Ruiz-Cañas
12,13
,
Bruno Sainz, Jr.
3,12,13
,
Ana Torres
,
Bozena Smolkova
,
Julie Earl
2,3
1
Molecular Epidemiology and Predictive Tumor Markers Group, Area 3
,
Ramón y Cajal Health Research Institute (IRYCIS)
,
2
The Biomedical Research Network in Cancer (CIBERONC)
,
3
Biobank and Biomodels Platform (PT20/0045), ISCIII research and development platforms in biomedicine and health sciences, BioBank Hospital Ramón y Cajal-IRYCIS, Spanish National Biobanks Network (ISCIII Biobank Register No. B.0000678)
,
Ramón y Cajal Health Research Institute (IRYCIS)
,
4
Faculty of Medicine
,
University of Alcalá de Henares
,
5
Institute of Tissue Medicine and Pathology
,
University of Bern
,
6
Department of Molecular Oncology, Cancer Research Institute
,
Biomedical Research Center of the Slovak Academy of Sciences
,
7
Experimental Oncology, Molecular Oncology Program
,
Centro Nacional de Investigaciones Oncológicas (CNIO)
,
8
Department of Surgical Oncology, National Cancer Institute
,
Slovak Medical University
,
9
Pancreatic and Biliopancreatic Surgery Unit
,
Hospital Universitario Ramón y Cajal
,
10
Department of Pathology
,
Hospital Universitario Ramón y Cajal
,
11
Department of Radiation Oncology
,
Hospital Universitario Ramón y Cajal
,
12
Department of Cancer
,
Instituto de Investigaciones Biomédicas “Alberto Sols” (IIBM)
,
13
Cancer Stem Cell and Fibroinflammatory Group, Chronic Diseases and Cancer, Area 3
,
IRYCIS
Tags
Keywords: Tumor Organoids
Primary Fibroblasts
DMEM/F-12 Media
PBS
Digestion Media
Trypsin
Cell Filtration
Cell Centrifugation
Red Blood Cell Lysis
Cell Dissociation
Cell Resuspension
Membrane Matrix
check_url/200204?article_type=t
Play Video
PDF
DOI
Cite This Article
Fresh Primary Tumor Tissue Processing to Establish Tumor Organoids and Primary Fibroblasts.
J. Vis. Exp.
(Pending Publication), e200204, doi:10.3791/200204 (2023).
Copy Citation
Download Citation
Reprints and Permissions
View Video
✖
To prove you're not a robot, please enter the text in the image below
Email:
Enable Javascript for audio controls
Enter Captcha text here:
Submit