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Biology
从细菌培养物中分离重组囊泡以释放可溶性蛋白
Published: June 30, 2023
doi:
10.3791/200248
Bree R. Streather
,
Karen Baker
,
Tara A. Eastwood
,
Daniel P. Mulvihill
1
School of Biosciences
,
University of Kent
Tags
Keywords: Recombinant Vesicle
Bacterial Culture
Soluble Protein
VNP Sequence Tag
Lysogeny Broth
Terrific Broth
IPTG
Centrifugation
Filtration
Vesicle Concentration
Sonication
BL21DE3 Escherichia Coli
MNeongreen
SDS-PAGE
check_url/200248?article_type=t
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Recombinant Vescicle Isolation from Bacterial Culture to Release Soluble Protein.
J. Vis. Exp.
(Pending Publication), e200248, doi:10.3791/200248 (2023).
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