Passaging and Scoring the <em>Caenorhabditis elegans</em> P0 Generation for Germline GFP Expression

doi:10.3791/200307

JoVE Journal > Genetics

Genetics

Passagem e Pontuação da Geração P0 de Caenorhabditis elegans para Expressão de GFP Germinativa

Published: May 05, 2023

doi:

10.3791/200307

Chengyin Li*¹, Phoebe A. W. Bhagoutie*¹, Victor Lao, Arneet L. Saltzman

¹Department of Cell and Systems Biology,University of Toronto

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P0 Generation Caenorhabditis Elegans Germline GFP Expression Agarose Pads Levamisole Worm Mounting Embryo Isolation Bleaching Protocol NGM Plates OP50-1 Bacteria Fluorescent Stereoscope GFP RNAi Transgene Silencing F1 Generation F2 Generation F5 Generation F10 Generation

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Passaging and Scoring the Caenorhabditis elegans P0 Generation for Germline GFP Expression. J. Vis. Exp. (Pending Publication), e200307, doi:10.3791/200307 (2023).

Passagem e Pontuação da Geração P0 de Caenorhabditis elegans para Expressão de GFP Germinativa

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