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Biology
次世代シーケンシングのためのポリ(A)RNA精製と重亜硫酸塩変換
Published: July 07, 2023
doi:
10.3791/200389
Szu-Ying Chen
2
,
Po-Hsien Huang
2
1
Institute of Basic Medical Sciences, College of Medicine
,
National Cheng Kung University
,
2
Department of Biochemistry and Molecular Biology, College of Medicine
,
National Cheng Kung University
Tags
Poly(A) RNA Purification
Bisulfite Conversion
Next-generation Sequencing
Total RNA
Oligo(dT) Beads
Lysis Buffer
Wash Buffer
Tris-HCl
RNA Elution
Magnetic Separation
RNA Sample Preparation
RNA Enrichment
check_url/200389?article_type=t
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Poly(A) RNA Purification and Bisulfite Conversion for Next Generation Sequencing.
J. Vis. Exp.
(Pending Publication), e200389, doi:10.3791/200389 (2023).
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