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Biology
Görüntüleme için maya hücrelerinin hazırlanması, görüntüleme koşullarının optimizasyonu ve görüntü toplama
Published: June 02, 2023
doi:
10.3791/200471
Emily Jie-Ning Yang
,
Istvan R. Boldogh
2
,
Haojie Ji
,
Liza Pon
2
,
Theresa C. Swayne
2
1
Department of Pathology and Cell Biology
,
Columbia University Irving Medical Center
,
2
Confocal and Specialized Microscopy Shared Resource in the Herbert Irving Comprehensive Cancer Center
,
Columbia University Irving Medical Center
Tags
Yeast Cells
Mitochondria
HyPer7
Microscopy
Imaging
Fluorescence
Confocal Microscopy
SCMOS Camera
Binning
Laser Power
Exposure
Dynamic Range
Noise Level
Time-lapse
Z-stacks
Transmitted Light
check_url/200471?article_type=t
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Preparation of Yeast Cells for Imaging, Optimization of Imaging Conditions, and Image Collection.
J. Vis. Exp.
(Pending Publication), e200471, doi:10.3791/200471 (2023).
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