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JoVE Journal > Neuroscience
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Neuroscience
Op liberase gebaseerde enzymatische zuivering van microgliacellen geïsoleerd uit het ruggenmerg van een muis
Published: September 22, 2023
doi:
10.3791/200616
Claudia I Gutiérrez-Román
2
,
María E Meléndez Camargo
,
Cuauhtémoc C García Rojas
,
Miguel Jimenez Olvera
,
Sandra H Gutiérrez Román
,
Oscar Medina-Contreras
1
Epidemiology, Endocrinology & Nutrition
,
Mexico Children’s Hospital
,
2
Pharmacy Department, National School of Biological Sciences
,
National Polytechnic Institute
,
3
Graduate Department
,
Dr. Eduardo Liceaga General Hospital of Mexico
,
4
Pain Clinic and Palliative Care
,
Dr. Eduardo Liceaga General Hospital of Mexico
,
5
General Surgery Service
,
Metropolitan Angeles Hospital
Tags
Microglia Isolation
Spinal Cord
Enzymatic Digestion
Liberase
Density Gradient
Cell Purification
FACS Analysis
check_url/200616?article_type=t
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Cite This Article
Liberase-Based Enzymatic Purification of Microglial Cells Isolated from a Mouse Spinal Cord.
J. Vis. Exp.
(Pending Publication), e200616, doi:10.3791/200616 (2023).
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