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JoVE Journal > Bioengineering
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Bioengineering
क्लोरेला वल्गरिस
का एग्रोबैक्टीरियम-मध्यस्थता परिवर्तन और कॉलोनी पीसीआर (सीपीसीआर) के माध्यम से जीन एकीकरण की पुष्टि
Published: October 27, 2023
doi:
10.3791/200638
M. Reza Roushan
,
Chuchi Chen
,
Parisa Ahmadi
,
Valerie C. A. Ward
1
Department of Chemical Engineering
,
University of Waterloo
Tags
Agrobacterium-mediated Transformation
Chlorella Vulgaris
Colony PCR (cPCR)
Gene Integration
Log Phase Culture
TAP Agar
Supplemented LB Broth
Agrobacterium Tumefaciens
Induction Medium
Selective Media
Tetracycline
DNA Agarose Gel
check_url/200638?article_type=t
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Agrobacterium-Mediated Transformation of
Chlorella vulgaris
and Confirmation of Gene Integration via Colony PCR (cPCR).
J. Vis. Exp.
(Pending Publication), e200638, doi:10.3791/200638 (2023).
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