JoVE Journal > Bioengineering

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클로렐라 심가리스(Chlorella vulgaris)의 아그로박테리움 매개 형질전환 및 콜로니 PCR(cPCR)을 통한 유전자 통합 확인

Published: October 27, 2023

doi:

M. Reza Roushan, Chuchi Chen, Parisa Ahmadi, Valerie C. A. Ward

¹Department of Chemical Engineering,University of Waterloo

Tags

Agrobacterium-mediated Transformation Chlorella Vulgaris Colony PCR (cPCR)Gene Integration Log Phase Culture TAP Agar Supplemented LB Broth Agrobacterium Tumefaciens Induction Medium Selective Media Tetracycline DNA Agarose Gel

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Agrobacterium-Mediated Transformation of Chlorella vulgaris and Confirmation of Gene Integration via Colony PCR (cPCR). J. Vis. Exp. (Pending Publication), e200638, doi:10.3791/200638 (2023).

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