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JoVE Journal > Cancer Research
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Cancer Research
הכנת צלחת להדמיית פלורסנט והערכת כדאיות התא בתרבית משותפת CAR-Jurkat עם תאי גידול המסומנים ב- CFSE
Published: October 27, 2023
doi:
10.3791/200793
Siddharth Subham*
1,2,3
,
John D. Jeppson*
1
,
Benjamin K. Gibbs
,
Jacqueline Babai
,
Riza Alker
,
Andrew K. Godwin
5,6
,
David Akhavan
2,3
1
Department of Radiation Oncology
,
University of Kansas Cancer Center
,
2
Department of Cancer Biology
,
University of Kansas Cancer Center
,
3
BioEngineering Program
,
University of Kansas
,
4
Department of Pathology and Laboratory Medicine
,
University of Kansas Medical Center
,
5
University of Kansas Cancer Center
,
6
Kansas Institute for Precision Medicine
,
University of Kansas Medical Center
Tags
Keywords: Fluorescent Imaging
Cell Viability
CAR-Jurkat Co-culture
CFSE-labeled Tumor Cells
Propidium Iodide
Triton X
Cell Imaging
CFSE Fluorescence
Cell Diameter
Live/dead Cell Analysis
Cell Counting
Effector-to-target Ratio
EGFR-targeting CAR
Cytotoxicity
PBMC-derived CD3 T Cells
check_url/200793?article_type=t
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Preparation of Plate for Fluorescent Imaging and Cell Viability Assessment in CAR-Jurkat Co-Culture with CFSE-Labeled Tumor Cells.
J. Vis. Exp.
(Pending Publication), e200793, doi:10.3791/200793 (2023).
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