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Biology
Transfection, Isolation, and Screening of the
CENP-E
Knockout HeLa Cells
Published: June 23, 2023
doi:
10.3791/201002
Meng-Fei Xu
2
,
Jie Chen
2
,
Yue Xu
2
,
Jing-Lian Zhang
2
,
Yi Zhou
2
,
Jie-Jie He
2
,
Shan Wu
2
,
Ya-Lan Wei
4
,
Zhen-Yu She
2
1
Department of Cell Biology and Genetics, The School of Basic Medical Sciences
,
Fujian Medical University
,
2
Key Laboratory of Stem Cell Engineering and Regenerative Medicine
,
Fujian Province University
,
3
College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics
,
Fujian Medical University
,
4
Medical Research Center
,
Fujian Maternity and Child Health Hospital
Tags
Keywords: Transfection
HeLa Cells
CENP-E Knockout
Plasmid
Trypsin-EDTA
Cell Passage
Reduced Serum Medium
Transfection Reagent
Fluorescence Microscopy
Cell Counting
Serial Dilution
Phenotype-based Screening
DNA Extraction
PCR
Ligation
Transformation
Sanger Sequencing
Wild-type
Mutant
check_url/201002?article_type=t
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Transfection, Isolation, and Screening of the
CENP-E
Knockout HeLa Cells.
J. Vis. Exp.
(Pending Publication), e201002, doi:10.3791/201002 (2023).
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