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Biology
Immunofluorescence Staining and High-Resolution Confocal Microscopy of CRISPR/Cas9-Mediated
CENP-E
Knockout HeLa Cells
Published: June 23, 2023
doi:
10.3791/201003
Meng-Fei Xu
2
,
Jie Chen
2
,
Yue Xu
2
,
Jing-Lian Zhang
2
,
Yi Zhou
2
,
Jie-Jie He
2
,
Shan Wu
2
,
Ya-Lan Wei
4
,
Zhen-Yu She
2
1
Department of Cell Biology and Genetics, The School of Basic Medical Sciences
,
Fujian Medical University
,
2
Key Laboratory of Stem Cell Engineering and Regenerative Medicine
,
Fujian Province University
,
3
College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics
,
Fujian Medical University
,
4
Medical Research Center
,
Fujian Maternity and Child Health Hospital
Tags
Immunofluorescence Staining
Confocal Microscopy
CRISPR/Cas9
CENP-E
HeLa Cells
Cell Fixation
Cell Permeabilization
Primary Antibody
Secondary Antibody
DAPI
Chromosome Misalignment
Metaphase
Interphase
Prophase
Anaphase
Telophase
check_url/201003?article_type=t
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Immunofluorescence Staining and High-Resolution Confocal Microscopy of CRISPR/Cas9-Mediated
CENP-E
Knockout HeLa Cells.
J. Vis. Exp.
(Pending Publication), e201003, doi:10.3791/201003 (2023).
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