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In Vitro Culturing of Environmental Sediment Sample for the Growth of Anaerobic Microorganisms

Published: January 12, 2024

doi:

Tia Zimmerman, Garion Leamon, Georgia Dillenburg, Bethany Egge, Jennifer Pierce, Ben Elliott, Trevor Murphy, Marjorie Brooks, Scott D. Hamilton-Brehm

¹Department of Microbiology,Southern Illinois University Carbondale, ²MEDPREP,Southern Illinois University School of Medicine, ³Department of Geology,Southern Illinois University Carbondale, ⁴Department of Zoology,Southern Illinois University Carbondale

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Keywords: In Vitro Culturing Environmental Sediment Sample Anaerobic Microorganisms Nitrogen Ethanol Syringe Needle Inoculation Culture Bottle Media Incubation Cell Counts Next-generation Sequencing (NGS)Geobacillus BLM-1 Inoculum

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In Vitro Culturing of Environmental Sediment Sample for the Growth of Anaerobic Microorganisms. J. Vis. Exp. (Pending Publication), e201271, doi:10.3791/201271 (2024).

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