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JoVE Journal > Immunology and Infection
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Immunology and Infection
Procedimento de Citometria de Fluxo para Medição do pH Relativo do Glicossomo em
Trypanosoma brucei
Vivo
Published: January 19, 2024
doi:
10.3791/201369
Daniel Call*
1
,
Sabrina S. Pizarro*
2,3
,
Erica Tovey
,
Emily Knight
3
,
Carrie Baumgardner
4
,
Kenneth A. Christensen
,
James C. Morris
1
Department Chemistry and Biochemistry
,
Brigham Young University
,
2
Eukaryotic Pathogens Innovation Center
,
Clemson University
,
3
Department of Genetics and Biochemistry
,
Clemson University
,
4
Department of Physics and Astronomy
,
Clemson University
Tags
Flow Cytometry
Glycosome
PH
Trypanosoma Brucei
PHluorin
Propidium Iodide
Thiazole Red
Forward Scatter
Side Scatter
Relative Fluorescence Intensity
check_url/201369?article_type=t
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DOI
Cite This Article
Flow Cytometry Procedure to Measure Relative pH of the Glycosome in Living
Trypanosoma brucei
.
J. Vis. Exp.
(Pending Publication), e201369, doi:10.3791/201369 (2024).
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