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JoVE Journal > Medicine
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Medicine
생체 내 연구를 위한 아데노 관련 바이러스를 정제하기 위한 이중 요오딕사놀 원심분리
Published: February 09, 2024
doi:
10.3791/201469
Kathryn K. Harris
,
Oleksandr Kondratov
,
Sergei Zolotukhin
1
Department of Pediatrics
,
University of Florida
Tags
Keywords: Adeno-associated Virus
AAV
Iodixanol
Ultracentrifugation
Purification
In Vivo Studies
Virus Clarification
Density Gradient
Centrifugation
Peristaltic Pump
Virus Fraction
15% Iodixanol
25% Iodixanol
40% Iodixanol
60% Iodixanol
Needle Aspiration
Virus Dilution
Lysis Buffer
check_url/201469?article_type=t
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Double Iodixanol Centrifugation to Purify Adeno-Associated Virus for In Vivo Studies.
J. Vis. Exp.
(Pending Publication), e201469, doi:10.3791/201469 (2024).
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