Transformation of E. coli Cells for a Complementation Assay to Assess Hsp70 Chaperone Activity

JoVE Journal > Biochemistry

Biochemistry

Transformasjon av E. coli-celler for en komplementeringsanalyse for å vurdere Hsp70 Chaperone-aktivitet

Published: March 08, 2024

doi:

10.3791/201542

Hloniphani Rachel Ncube, Unarine Dali, Tendamudzimu Harmfree Dongola, Addmore Shonhai

¹Department of Biochemistry & Microbiology,University of Venda

Tags

Keywords: E. Coli DnaK756 Complementation Assay Hsp70 Chaperone Activity Heat Shock Plasmid DNA Transformation Agar Plates Antibiotic Selection Serial Dilutions Permissive And Non-permissive Growth Temperatures Recombinant Protein Expression KPf KPf-V436F

Play Video

PDF

DOI

Cite This Article

Transformation of E. coli Cells for a Complementation Assay to Assess Hsp70 Chaperone Activity. J. Vis. Exp. (Pending Publication), e201542, doi:10.3791/201542 (2024).

Transformasjon av E. coli-celler for en komplementeringsanalyse for å vurdere Hsp70 Chaperone-aktivitet

Tags

Play Video

Cite This Article

View Video

✖

To prove you're not a robot, please enter the text in the image below