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Biochemistry
Transformation of
E. coli
Cells for a Complementation Assay to Assess Hsp70 Chaperone Activity
Published: March 08, 2024
doi:
10.3791/201542
Hloniphani Rachel Ncube
,
Unarine Dali
,
Tendamudzimu Harmfree Dongola
,
Addmore Shonhai
1
Department of Biochemistry & Microbiology
,
University of Venda
Tags
Keywords: E. Coli
DnaK756
Complementation Assay
Hsp70 Chaperone Activity
Heat Shock
Plasmid DNA
Transformation
Agar Plates
Antibiotic Selection
Serial Dilutions
Permissive And Non-permissive Growth Temperatures
Recombinant Protein Expression
KPf
KPf-V436F
check_url/201542?article_type=t
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Transformation of
E. coli
Cells for a Complementation Assay to Assess Hsp70 Chaperone Activity.
J. Vis. Exp.
(Pending Publication), e201542, doi:10.3791/201542 (2024).
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