UV Irradiation: A Method to Integrate Extrachromosomal Arrays

Abstract

Source: Mariol, M. C et al. A Rapid Protocol for Integrating Extrachromosomal Arrays With High Transmission Rate into the C. elegans Genome. J. Vis. Exp. (2013).

Microinjection of transgenes usually results in C. elegans strains with large extrachromosomal arrays, which result in variable expression and transmission of the transgene.  UV Irradiation can induce chromosomal integration of the array and more stable transgene transmission.

Protocol

This protocol is an excerpt from Mariol et al, A Rapid Protocol for Integrating Extrachromosomal Arrays With High Transmission Rate into the C. elegans Genome, J. Vis. Exp. (2013). 1. UV Irradiation and Recovery of Transgenic Worms Worm irradiation: Pick 30-100 fluore…

Representative Results

Table 1. Comparison of the standard and improved protocols for extrachromosomal arrays integration into the C. elegans genome. Two nonintegrated transgenes transmitting at about the same frequency were integrated using the standard or the improved protocol. The main difference lies in the fact that in the standard protocol a screen of the proge…

Materials

U.V. cross-linker	Fischer Scientific		Wavelength 254 nm
Microscope SteREO Discovery V8	Carl Zeiss, Inc.
Petri dishes, 60 mm	CML	BPS55E6
Platinium wire 0.25 mm	Alfa Aesar	6/4/7440	To make a pick for worms