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JoVE Journal > Bioengineering
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Bioengineering
Produzione di AAV ricombinante mediante trasfezione transitoria
Published: April 05, 2024
doi:
10.3791/201698
Miguel M. Lopes*
1,2,3
,
Sara M. Lopes*
1,2,3
,
Rafael Baganha
2,4
,
Carina Henriques
2,5
,
Ana C. Silva
2,3
,
Diana D. Lobo
2,3
,
Luísa Cortes
2,3,6
,
Luís Pereira de Almeida*
1,2,4,5
,
Rui Jorge Nobre*
1,2,3,4
1
CNC-UC – Center for Neuroscience and Cell Biology
,
University of Coimbra
,
2
CIBB – Center for Innovative Biomedicine and Biotechnology
,
University of Coimbra
,
3
IIIUC – Institute for Interdisciplinary Research
,
University of Coimbra
,
4
ViraVector – Viral Vectors for Gene Transfer Core Facility
,
University of Coimbra
,
5
FFUC – Faculty of Pharmacy
,
University of Coimbra
,
6
MICC-CNC – Microscopy Imaging Center of Coimbra – CNC
,
University of Coimbra
Tags
Recombinant AAV
Transient Transfection
HEK293T Cells
Cell Culture
Cell Transfection
Polyethyleneimine
DMEM
Cell Harvesting
Cell Lysis
Benzonase Nuclease
Centrifugation
Filtration
check_url/201698?article_type=t
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Production of Recombinant AAV by Transient Transfection.
J. Vis. Exp.
(Pending Publication), e201698, doi:10.3791/201698 (2024).
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