Extracting Intact Kidney from Mouse: A Technique to Obtain Kidney Without Renal Capsule from Murine Models
Published: April 30, 2023
Abstract
Source: Grainger, N. et al. Isolating and Imaging Live, Intact Pacemaker Regions of Mouse Renal Pelvis by Vibratome Sectioning. J. Vis. Exp. (2021)
In this video, we describe the procedure for kidney extraction from the retroperitoneal space of the mouse. The extracted kidney can further be microdissected to obtain an intact kidney without a renal capsule and the surrounding fat layer.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Kidney Dissection Anaesthetize mice by inhalation of 3–4% isoflurane in a ventilated hood. Confirm the induction of deep anesthesia by loss of toe and/or tail pinch reflex and then euthanize the mice by cervical dislocation. Apply 70% ethanol to the chest to dampen the fur. Using…
Representative Results
Figure 1: Basic kidney anatomy and location of PKJ pacemaker region. (A) Diagram of the intact kidney showing the orientation of the RP and ureter. The renal artery and renal vein are displayed in red and blue, respectively. (B) The intact kidney can be cut along a sagittal plane to expose the inner aspect of the kidney, including the medulla, papilla (dista…
Disclosures
The authors have nothing to disclose.
Materials
| 60 mm x 15 mm Petri dish | Sigma Aldrich | P5481 | Kidney sharp dissection dish |
| Absorbent paper | Fisher Scientific | 06-666A | To dry the kidney before applying glue |
| B6;129S-Gt(ROSA)26Sor/J | The Jackson Laboratory | 13148 | GCaMP3 Mice |
| B6;129S-Gt(ROSA)26Sor/J | The Jackson Laboratory | 24105 | GCaMP6f Mice |
| B6.FVB-Tg(Myh11-cre/ERT2)1Soff/J | The Jackson Laboratory | 19079 | smMHC-CRE Mice |
| C57BL/6-Tg(Pdgfra-cre)1Clc/J | The Jackson Laboratory | 13148 | PDGFRa-CRE Mice |
| Ethanol | Phamco-Aaper | SDA 2B-6 | For dissection |
| Extra-fine Bonn Scissors | Fine Science Tools | 14083-08 | Used for internal dissecting scissors |
| Fine scissors | Fine Science Tools | 14060-09 | Used for external dissecting scissors |
| Fine-tip forceps | Fine Science Tools | 11254-20 | Used for fine dissection of kidney |
| Isoflurane | Baxter | NDC 1001936060 | For anesthesia |
| Silicon elastomer | Fisher Scientific | NC9285739 | Sylgard 184 |
| Student Adson Forceps | Fine Science Tools | 91106-12 | For gently holding and moving the kidney |
| Student Dumont Forceps | Fine Science Tools | 91150-20 | Used for internal dissecting forceps |
| Vannas spring scissors | Fine Science Tools | 15000-03 | For sharp dissection and cleanup of isolated kidney |
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Cite This Article
Extracting Intact Kidney from Mouse: A Technique to Obtain Kidney Without Renal Capsule from Murine Models. J. Vis. Exp. (Pending Publication), e20549, doi: (2023).