失血性休克模型已被一个可靠和可再生资源,促进与炎症和创伤后器官损害相关的信号级联反应的鉴定和了解。本文提供了相关的手术失血性休克小鼠实验过程和机械方面的的一步一步的说明。
这是常识,严重失血和外伤导致的不利信号事件往往造成的死亡率级联1,2,3,4,5,这些信号事件也可能导致败血症和/或多个器官功能障碍(按付款当日价格计算)6,7,8,9,10是至关重要的,然后进行调查,以便制定更有效的方法,以帮助遭受创伤的患者的免疫功能受到抑制和不利的信号级联的原因。这种固定式压力失血性休克(HS)程序,虽然在技术上具有挑战性,是一个优秀资源调查这些病理生理条件,11,12,13在生物系统评估的进展,即系统生物学使科学界进一步了解复杂的生理网络和蜂窝通信模式14。失血性休克已被证明是一个揭幕这些蜂窝通信模式,因为这涉及到免疫功能的重要工具,15,16,17,18,可以掌握此过程!此过程也可以被用来作为一个固定的数量或固定压力的方法。我们适应这种技术在小鼠模型,以加强在先天免疫和适应性免疫功能的研究,19,20,21,由于他们在小鼠体积小房协提出了独特的挑战。然而,由于许多可用的小鼠品系,这个物种的代表为研究的生物反应空前资源。 HS模型是一个重要的模型研究蜂窝移动通信的模式和系统,如激素和炎症介质系统,危险的信号,即阻尼和PAMP上调的反应,因为它会引起其他形式的冲击,从不同的不同的反应 。22,23 ,24,25转基因小鼠品系的开发和诱导生物制剂来抑制特定的信号,提出了宝贵的的机会,进一步阐明我们了解向上和向下调控的信号转导后出现严重失血,即HS和创伤26,27 ,28,29,30。
有众多的复苏方法与HS和创伤协会(R) 的31,32,33,34只乳酸林格氏液(LR),等于流血量的三倍,固定体积的复苏方法是在此模型中使用研究远程器官损伤和全身性炎症,如内源性机制,35,36,38这复苏的方法被证明是有效评估HS 和创伤38,39影响。
The authors have nothing to disclose.
资金来源/编号分子生物学失血性休克GM053789
Instrument | Manufacturer | Catalogue Number |
---|---|---|
Dumonts(SS/45° angle 0.2×0.12mm-tip, 13.5cm length) | Fine Science Tools | 11203-25 |
Surgical scissors (straight-12cm) | Fine Science Tools | 14068-12 |
Hemostats (curved-12.5cm) | Fine Science Tools | 13009-12 |
Microscissors (spring scissors- straight-8cm) | Fine Science Tools | 15000-00 |
Forceps (0.8mm-tip, curved-10cm) | Fine Science Tools | 11050-10 |
suture reel 6-0 | Fine Science Tools | 18020-60 |
suture 4-0 PDSII | Penn Vet | ETHZ304H |
gauze 4×4 | can be purchased through any global vendor | |
cotton-tip applicator | can be purchased through any global vendor | |
30G needle | can be purchased through any global vendor | |
23G needle | can be purchased through any global vendor | |
3cc syringe | can be purchased through any global vendor | |
5cc syringe | can be purchased through any global vendor | |
10cc syringe | can be purchased through any global vendor | |
50cc conical tube | can be purchased through any global vendor | |
1cc syringe w/ 25G needle | Fisher Scientific | 14-826-88 |
Polyethylene 10 tubing 100` (PE-10) | Fisher Scientific | 14-170-12P |
Polyethylene 50 tubing 100` (PE-50) | Fisher Scientific | 14-170-12B |
3-way stopcock | Fisher Scientific | NC9779127 |
surgical blue pad | Fisher Scientific | 50-7105 |
Sterile Field dressings | Fisher Scientific | NC9517505 |
tape rolls 1″ | Corporate Express | MMM26001 |
straight side wide mouth jars (used as cap for nose cone) | VWR | 159000-058 |
stainless steel tray 8″ x 11″ | VWR | 62687-049 |
male-male leur lock 3-way | VWR | 20068-909 |
sterilization pouch 3″x8″ | VWR | 24008 |
sterilization pouch 5″x10″ | VWR | 24010 |
Wild M650 microscope w/ boom stand | Leica | |
Leica IC D digital camera/live image | Leica | |
Digi-Med BPA-400 analyzer & systems integrator | Micro-Med | SYS-400 |
TXD-310 (Digi-Med Transducer) | Micro-Med | TXD-300 |
Computer | Dell | |
Hot bead instrument sterilizer | VWR | 18000-45 |
Oster A5 clippers w. size 40 blade | VWR | 10749-020 |
circulating heating pad 18×26 | Harvard | py872-5272 |
rectal thermometer | Kent Scientific | RET-3 |
Reagent | Concentration | Manufacturer | Catalogue Number |
---|---|---|---|
Pentobarbital Sodium (Nembutol Sodium Solution) | 70mg/kg | Ovation | |
Buprenorphine HCl | 0.1mg/kg | Bedford Laboratories | |
Lactated Ringers Injection 250cc IV bag | Baxter | ||
Aerrane (Isoflurane) | 99.9% | NLS Animal Health | 105996 |
Heparin Sodium | 1000U | Abraxis | |
Bacteriostatic Sodium Chloride | 0.9% | Hospira | |
Ethyl Alcohol | 70% | Pharmaco-AAPER | |
Triadine Povidone Iodine (Betadine) | Triad disposables |