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Organotypischen Kultur der GFP-exprimierenden Maus Embryonen für Real-time Imaging der peripheren Nerven Outgrowth
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Organotypic Slice Culture of GFP-expressing Mouse Embryos for Real-time Imaging of Peripheral Nerve Outgrowth
DOI:

05:42 min

March 29, 2011

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Chapters

  • 00:05Title
  • 01:20Preparation for Slicing and Culturing
  • 02:08Embryo Embedding
  • 03:18Slicing Procedure
  • 04:23Imaging Spinal Nerve Outgrowth
  • 04:54Representative Results of Imaging Spinal Nerve Outgrowth
  • 05:21Conclusion

Summary

Automatic Translation

Wir präsentieren eine Methode zur organotypischen Schnitten der Mitte der Schwangerschaft Maus-Embryonen für die Kultivierung und Zeitraffer-Bildgebung von peripheren Nerven Auswuchs vorzubereiten.

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