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V3的HIV - 1取向的基因型推理使用人口为基础的测序
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
Genotypic Inference of HIV-1 Tropism Using Population-based Sequencing of V3
DOI:

11:10 min

December 27, 2010

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Chapters

  • 00:05Title
  • 01:25Amplification of HIV cDNA from Blood Plasma
  • 03:37Gel Electrophoresis
  • 04:29Sequencing
  • 07:18Data Analysis with Base Calling Software
  • 08:18The Geno2pheno Co-receptor Algorithm
  • 09:37Interpreting the Geno2pheno FPR
  • 10:37Conclusion

Summary

Automatic Translation

可以推断出艾滋病毒嗜性病毒包膜V3区。 V3是一式三份,采用巢式RT - PCR扩增,测序,PCR扩增,并解释使用生物信息学软件。被列为非R5病毒与G2P分数低1个或多个序列(S)的样品。

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