Materials
Name | Company | Catalog Number | Comments |
(1) Tissue culture incubator (H) | |||
(1) Heat-resistant plastic enclosure (A) | (delrin or comparable) | ||
(1) Plastic frame for 35mm GBM lid (M) | (for affixing membrane to petri dish) | ||
(1-2) linear ft. Clear PFTE (Teflon) | membrane material | ||
(4) Brass thumb screws | |||
(2) Small 10kOhm heatsink resistors | used as heating elements inside stage-top incubator enclosure | ||
(1) Transformer | supply of 9V current to resistors | ||
(1) Terrarium temperature controller and probe | thermostatic regulation of power to heatsink resistors via transformer | ||
(1) 2000ml Erlenmeyer flask (K) | muffler for vibration suppression in hose before stage-top incubator | ||
(1) 1/8" sorbothane sheet | gasket material for base of stage-top incubator | ||
(1) Airtight ABS (or comparable) plastic box (J) | housing for aquarium pump | ||
(1) Aquarium pump (I) | |||
(1) Small computer cooling fan | |||
(1) Plastic enclosure for cooling fan (L) | |||
(1) 9V transformer for computer cooling fan | |||
(20-30ft) Nalgene or silicone hose (F,G,N) | connections between tissue culture and stage-top incubators; moisture traps | ||
(2) Plastic stopcocks (O) | to open and close air flow before and after stage-top incubator | ||
(4) Barbed brass hose connectors | for hose connections to/from stage-top incubator and aquarium pump enclosure | ||
(2) Brass quick couplers | for hose connections to/from stage-top incubator | ||
(2) Brass quick couplers | for hose connections to/from stage-top incubator | ||
Table 1. Stage-top incubator parts: | |||
Poly-D-lysine | Sigma-Aldrich | P7280-5MG | |
laminin | Roche Group | 11243217001 | |
35 mm glass-bottom dishes | MatTek Corp. | P35GC-0-14-C | |
DMEM | Life Technologies | 10567 | |
B27 | Life Technologies | 17504-044 | |
Glutamax | Life Technologies | 35050 | |
Lipid-rich BSA | Life Technologies | 11020-021 | |
L-Asparagine | Sigma-Aldrich | P0380-100G | |
L-Proline | Sigma-Aldrich | A8381-100G | |
Vitamin B-12 | Sigma-Aldrich | V2876-100MG | |
5-HT | Sigma-Aldrich | H9523-25MG | |
8-OH-DPAT | Sigma-Aldrich | H8520-25MG | |
Dopamine | Sigma-Aldrich | H8502-5G | |
Bromocriptine | Sigma-Aldrich | B2134-25MG | |
SKF38393 | Sigma-Aldrich | D047-100MG |
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