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跨膜结构域齐聚倾向ToxR含量决定
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JoVE Journal Biology
Transmembrane Domain Oligomerization Propensity determined by ToxR Assay
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跨膜结构域齐聚倾向ToxR含量决定

DOI:
10.3791/2721-v

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06:45 min

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May 26, 2011

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, ,
1Department of Chemistry and Biochemistry,University of Colorado at Boulder

Chapters

  • 00:05Title
  • 01:26Expression of the Chimeric Proteins
  • 02:30Measurement of β-Galactosidase Activity
  • 03:40Verification of Protein Expression
  • 04:24Control for Proper Membrane Insertion
  • 05:34Representative Results of the ToxR Transcriptional Reporter Assay
  • 06:17Conclusion

Summary

Automatic Translation

Automatic Translation

一个高效的程序，以评估单通跨膜结构域的寡聚化倾向（TMDS）描述。融合到ToxR的战区导弹防御系统组成的嵌合蛋白在大肠杆菌记者应变中表达。 TMD诱导齐聚的原因ToxR，记者​​蛋白的转录和生产，半乳糖苷酶的激活二聚体。

Tags

Transmembrane DomainOligomerization PropensityToxR AssayProtein Transmembrane DomainsPhospholipid BilayersMembrane-spanning ProteinsHydrophobic HelicesStructured Transmembrane OligomersAmino AcidsMembrane EnvironmentAqueous SolutionIntermolecular ForcesProtein AssociationMolecular RecognitionWater-soluble RegionsTransmembrane OligomerizationAssociation MeasurementIntegral Membrane Protein FunctionSynthetic PeptidesCellular Membranes

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