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Journal
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Immunology and Infection
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心脏功能免疫细胞的分离
JoVE Journal
Immunology and Infection
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Immunology and Infection
Isolation of Functional Cardiac Immune Cells
Please note that all translations are automatically generated.
Click here for the English version.
心脏功能免疫细胞的分离
DOI:
10.3791/3020-v
•
07:26 min
•
December 05, 2011
•
Jennifer L. McLarty
,
Giselle C. Meléndez
,
William J. Spencer
,
Scott P. Levick
,
Gregory L. Brower
,
Joseph S. Janicki
1
Department of Cell Biology and Anatomy
,
University of South Carolina- School of Medicine
Chapters
00:05
Title
01:31
Insertion of Tracheal Tube
02:51
Immune Cell Isolation
05:54
Representative Histological, Functional and Flow-cytometric Results of Cardiac Immune Isolated Cells
07:05
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这种隔离的心脏功能的免疫细胞的方法提供了一种替代传统的方法胶原酶消化,从而导致不必要的免疫细胞活化,从而导致了这些细胞的减少响应。我们隔离的方法,产生心脏功能的免疫细胞,避免与酶消化相关的问题。
Tags
Functional Cardiac Immune Cells
Pathological Remodeling
Cardiac Diseases
Mast Cells
T-cells
Macrophages
Biologically Active Mediators
Cytokines
Proteases
Tryptase
Extracellular Matrix Metabolism
Matrix Metalloproteinases
Collagen Accumulation
Cardiac Fibroblasts’ Function
Isolating Cardiac Immune Cells
Loss Of Function
Technique Development
Understanding Of Role In Cardiac Disease
Anatomical Location
Rat’s Xiphoid Process
Axilla
Falciform Ligament
Pericardium Of The Heart
Characterization Of Functionality
Phenotype
Maturity
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