Titel der Verkapselung von Zellen durch Tröpfchen
1Bio-Acoustic-MEMS Laboratory in Medicine (BAMM), HST-Center for Bioengineering, Brigham and Women’s,Harvard Medical School, 2Bio-Acoustic-MEMS Laboratory in Medicine (BAMM), HST-Center for Bioengineering,Brigham and Women’s Hospital, 3Brigham and Women’s Hospital,Harvard Medical School, 4Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology; Center for Biomedical Engineering, Department of Medicine,Brigham and Women’s Hospital
Protocol
NIH3T3-Zellen der Zubereitung: A. Zellen für Ejection Trypsinize Zellen, dann verdünnt 1:1 mit Zell-Medien und Übertragung von einem T75 Kolben in ein 15 ml Falcon-Röhrchen Spin down-Zellen zu einem Pellet durch Zentrifugation, Absaugen des Überstandes und waschen Sie die Zellen mit DPBS Spin down-Zellen zu einem Pellet erneut, und saugen Überstand Die Zellen in den Medien Bestimmen Sie die Zelldichte mit Zählkammer (~ 200 X 10 4</s…
Disclosures
The authors have nothing to disclose.
Materials
| Material Name | Type | Company | Catalogue Number | Comment |
|---|---|---|---|---|
| Fluorescent Microscope | Nikon | Eclipse TE-2000 U | ||
| Solenoid valve cell ejector | Operation frequency: 1 KHz, 30psi, 50nl~0.5ul. 12V Valve Driver: 2.5 Amp drive current | |||
| 5-Gallon Portable air tank | Coleman | Powermate CT5 | Pressurized air: 30 PSI | |
| Pressure regulators | Marsh Bellofram | |||
| Pulse Generator | HP8112A | Actuation frequency generation: 50 MHz | ||
| XYZ-stage | Newmark Systems | With Stepping motor: 6inch travel xy-stage(NLS4-6-25), 2.5inch travel z-stage(NLS4-2.5-25), 3axis controller(NSC-G3), 0.1um resolution | ||
| NIH 3T3 | Cell-line | fibroblasts | ||
| Trypsin | 0.05% solution | |||
| NIH 3T3 cell medium | ||||
| DPBS | Buffer | |||
| T75 | Tissue culture flasks | |||
| Plastic conical tubes | 15 ml, for tissue culture |
Cite This Article
Moon, S., Lin, P., Keles, H. O., Yoo, S., Demirci, U. Title Cell Encapsulation by Droplets. J. Vis. Exp. (8), e316, doi:10.3791/316 (2007).