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Chapters
- 00:05Title
- 02:46Isolation of Primary Cultured Human Normal Mammary Fibroblasts
- 04:43Generation of GFP-labeled, Puromycin-Resistant and Immortalized Human Normal Mammary Fibroblasts
- 07:35Co-injection of Human Mammary Fibroblasts with Breast Carcinoma Cells into an Immunodeficient Mouse
- 08:58Extraction of CAFs or their Corresponding Control Fibroblasts from the Tumor or Fibroblast Xenografts, Respectively
- 13:31Representative Images of Experimentally Generated CAFs
- 15:18Conclusion
Carcinoma associato fibroblasti (CAF), ricco di miofibroblasti presenti nello stroma del tumore, svolgono un ruolo importante nel guidare la progressione del tumore. Abbiamo sviluppato un tumore modello coimplantation xengraft per generare sperimentalmente CAF da fibroblasti umani mammaria. Il protocollo descrive come stabilire miofibroblasti CAF che acquisiscono la capacità di promuovere la tumorigenesi.
Tags
Carcinoma-associated FibroblastsCAFsHuman Mammary FibroblastsStromaExtracellular MatrixMesenchymal CellsFibroblastsMyofibroblastsEndothelial CellsPericytesLeukocytesTumour-associated StromaParacrine Signalsα-smooth Muscle Actin (α-SMA)Activated FibroblastsHigh-grade MalignanciesPoor PrognosesImmunodeficient MiceTumourigenesisSenescence
