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Bioengineering
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在胎儿肺细胞的机械传导的实验系统研究
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Bioengineering
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JoVE Journal
Bioengineering
An Experimental System to Study Mechanotransduction in Fetal Lung Cells
Please note that all translations are automatically generated.
Click here for the English version.
在胎儿肺细胞的机械传导的实验系统研究
DOI:
10.3791/3543-v
•
09:35 min
•
February 16, 2012
•
Yulian Wang
,
Zheping Huang
,
Pritha S. Nayak
,
Juan Sanchez-Esteban
1
Women & Infants Hospital of Rhode Island
,
Alpert Medical School of Brown University
Chapters
00:05
Title
00:58
Coating Culture Plates with Extra-cellular Matrix Proteins
02:15
Isolation of Fetal Rodent Lung Type II Epithelial Cells
05:32
Isolation of Fetal Rodent Lung Fibroblasts
06:15
Experimental System to Provided Mechanical Stimulation to Lung Cells
07:42
Representative Results from Mechanotransduction Experiments Using Fetal Lung Cells
09:15
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
机械力发挥关键作用,在肺发育和肺损伤。在这里,我们描述了一个隔离啮齿动物胎儿肺Ⅱ型上皮细胞和成纤维细胞,并揭露他们使用的机械刺激的方法<em>在体外</em>系统。
Tags
Experimental System
Mechanotransduction
Fetal Lung Cells
Mechanical Forces
Lung Development
Alveolar Type II Epithelial Cells
Pulmonary Surfactant
Fluid Homeostasis
Distal Lung Parenchyma Cells
Exaggerated Stretch
Mechanical Ventilation
Molecular Mechanisms
Cellular Mechanisms
Mechanical Stimuli
Lung Injury
Isolation Method
Flexcell Strain Unit
In Vitro System
Mechanical Stimulation
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