Journal
/
Medicine
/
Stereotactic Intracranial Implantation and In vivo Bioluminescent Imaging of Tumor Xenografts in a Mouse Model System of Glioblastoma Multiforme
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
Stereotactic Intracranial Implantation and In vivo Bioluminescent Imaging of Tumor Xenografts in a Mouse Model System of Glioblastoma Multiforme

Stereotactic Intracranial Implantation and In vivo Bioluminescent Imaging of Tumor Xenografts in a Mouse Model System of Glioblastoma Multiforme

DOI:
10.3791/4089-v

•

10:52 min

•

September 25, 2012

•

, , , ,
1Department of Radiation Oncology,University of Pennsylvania

Chapters

  • 00:05Title
  • 02:08Orthotopic Xenograft Implantation
  • 07:41Bioluminescent Imaging (BLI) to Monitor Tumor Growth and Response to Therapy
  • 08:38Results: In Vivo Bioluminescent Imaging of Tumor Xenografts in a Mouse Model System of Glioblastoma Multiforme
  • 09:52Conclusion

Summary

Automatic Translation

Automatic Translation

We describe an integrated method for the precise, stereotactic implantation of human glioblastoma multiforme cells into the brains of nude mice and subsequent serial in vivo imaging to monitor growth and response to treatment of the resultant xenografts.

Tags

Stereotactic Intracranial ImplantationIn Vivo Bioluminescent ImagingTumor XenograftsMouse Model SystemGlioblastoma MultiformeHigh-grade Primary Brain CancerMedian SurvivalStandard Tri-modality TreatmentSurgical ResectionRadiation TherapyTemozolomide ChemotherapyTherapeutic ApproachesAnimal ModelsTumor GrowthTreatment ResponseBio-imageable GBM Cancer CellsNude MiceAnatomic LocationsIn Vivo Bioluminescent ImagingIntracranial Xenograft GrowthPerioperative MorbidityMortality

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/4089?article_type=v

Multi-photon Imaging of Tumor Cell Invasion in an Orthotopic Mouse Model of Oral Squamous Cell Carcinoma
check_url/4089?article_type=v

In vivo Bioluminescence Imaging of Tumor Hypoxia Dynamics of Breast Cancer Brain Metastasis in a Mouse Model
check_url/4089?article_type=v

In vivo Dual Substrate Bioluminescent Imaging
check_url/4089?article_type=v

Intracranial Implantation with Subsequent 3D In Vivo Bioluminescent Imaging of Murine Gliomas
check_url/4089?article_type=v

Screening for Melanoma Modifiers using a Zebrafish Autochthonous Tumor Model
check_url/4089?article_type=v

Live Imaging of Drug Responses in the Tumor Microenvironment in Mouse Models of Breast Cancer
check_url/4089?article_type=v

A Novel High-resolution In vivo Imaging Technique to Study the Dynamic Response of Intracranial Structures to Tumor Growth and Therapeutics
check_url/4089?article_type=v

In vivo 19F MRI for Cell Tracking
check_url/4089?article_type=v

Creating Anatomically Accurate and Reproducible Intracranial Xenografts of Human Brain Tumors
check_url/4089?article_type=v

Intrauterine Telemetry to Measure Mouse Contractile Pressure In Vivo

Read Article