这在朱庇特一月

Summary

Abstract

可视化实验杂志“（朱庇特）开始至2012年出版其第1500条，其中自由激进的香烟烟雾成分分析方法的重点。虽然烟焦油被认为是香烟烟雾中的致癌剂，自由基是众所周知的致癌分子，而这些一级致癌物在香烟烟雾中，是目前已知。在这篇文章中采用的方法相结合的一个单端口的吸烟设备，模拟香烟吞云吐雾，电子自旋共振（ESR）光谱，技术，措施自由基的未成对电子发出的旋转能量，的使用。本文中使用的设备单端口吸烟的一个重要修改，是在前面的自旋捕集液氮陷阱的介绍。液态氮去除香烟烟雾中的水蒸气，大大提高了结果的ESR谱。

除了测量香烟烟雾中的自由基浓度，植物抗氧化剂被引入到修改过的香烟过滤嘴，并获得通过这些过滤器的香烟烟雾中的ESR谱。葡萄籽提取物，番茄红素，观察有最显著的清除自由基的作用，如果成本的有效手段，包括香烟过滤嘴，这些分子可以开发，可以创造危害较小的卷烟。

朱庇特的临床和转化医学，提出了一个用于确定本地Gyrification指数（LGI）的分析方法 – 皮质折叠程度的测量。皮质褶皱发展，在最初的几个产后个月，并在这段时间内的不良事件可导致大脑的表面解剖，最终可能与神经精神障碍相关的异常。

计算的本地Gyrification指数开发的分析工具是神父eesurfer，它可以转换成三维重建的皮质T1加权磁共振成像扫描。创建单独的重建是灰色/白色的母校和灰色/软脑膜接口，这是前computating的LGI手动调整。平滑操作之后，软脑膜和灰色/白色表面的圆形区域比较，以计算LGI在整个大脑的千点。 Freesurfer使得直接gyrification分析和临床医生和脑科学家，谁是在正常和疾病状态下的不同个体之间的比较皮质折叠程度有兴趣的。

朱庇特的免疫和感染月份的文章继续该杂志的出版载体出生疾病的研究前沿方法的传统。具体来说，本文将朱庇特的疟疾研究协议的全面收集，扩大我们的图书馆脑型疟疾 – 一种破坏性与儿童的高易感性的条件。简单地说，此协议涉及到培养皿中传代，一个人的大脑血管内皮细胞单层，暴露的恶性疟原虫感染的红细胞。以下步骤的清洗和丰富，产量高的寄生虫，它携带感染大脑的潜力，可以分离和进一步的特点。

在神经科学，朱庇特展示了研究神经嵴细胞的迁移的方法。这些细胞是一过性，多能，发展的细胞类型，除了成为神经组织，分化成平滑肌，嗜铬细胞，cartiliage，和骨。鸡胚精心准备，使躯干区域可以分离并切除过夜培养的神经管纤维连接蛋白涂层的盖玻片，使神经嵴细胞从神经管移民。去除神经管，盖玻片培养的神经嵴细胞被转移到一个修改Zigmond室 – 一腔，使跨文化成立一个公认的趋化剂的浓度梯度。 Timelapes成像其次是细胞运动轨迹的分析，是用来评估装入zigmund室分子趋化潜力，洞察提供了分子线索，这一重要的发展类型的细胞迁移指导。

这些功能的文章，包括五十四1月发布的视频协议。其他著名的文章，包括C.角质层结构可视化的方法线虫，热成像迟发性肌肉酸痛，培养腹脑器官切片，并诱导小鼠动脉粥样硬化。

Protocol

Selection of Plasmodium falciparum Parasites for Cytoadhesion to Human Brain Endothelial Cells Antoine Claessens, J. Alexandra RoweCentre for Immunity, Infection and Evolution, University of Edinburgh An in vitro model for cerebral malaria sequestration is described1. Plasmodium falciparum infected red blood cells are selected for binding to immortalized human brain microvascular endothelial cells. The selected parasites show a distinct phenotype. The selection process can be applied using various P. falciparum strains and endothelial cell lines. Implantation of a Carotid Cuff for Triggering Shear-stress Induced Atherosclerosis in Mice Michael T. Kuhlmann1, Simon Cuhlmann2, 3, Irmgard Hoppe1, Rob Krams3, Paul C. Evans2, Gustav J. Strijkers4, Klaas Nicolay4, Sven Hermann1, Michael Schäfers11European Institute for Molecular Imaging, Westfälische Wilhelms-University Münster, 2British Heart Foundation Cardiovascular Sciences Unit, Imperial College London , 3Department of Bioengineering, Imperial College London , 4Biomedical Engineering, Eindhoven University of Technology The constricting cuff presented in this article is designed to induce atherosclerosis in the murine common carotid artery. Due to the conical shape of its inner lumen the implanted cuff generates well-defined regions of low, high and oscillatory shear stress triggering the development of atherosclerotic lesions of different inflammatory phenotypes. Analysis of Trunk Neural Crest Cell Migration using a Modified Zigmond Chamber Assay Christopher C. Walheim1, Juan Pablo Zanin2, Maria Elena de Bellard11Department of Biology, California State University, Northridge, 2Centro de Biología Celular y Molecular, Universidad Nacional de Córdoba An approach to analyze the migration of explanted cells (trunk neural crest cells) is described. This method is inexpensive, gentle, and capable of distinguishing chemotaxis from both chemokinesis and other influences on migratory polarity such as those derived from cell-cell interactions within the primary trunk neural crest cell culture. Organotypic Slice Cultures of Embryonic Ventral Midbrain: A System to Study Dopaminergic Neuronal Development in vitro Gabriela Oana Bodea, Sandra BlaessInstitute of Reconstructive Neurobiology, University of Bonn A method to generate organotypic slices from the E12.5 murine embryonic midbrain is described. The organotypic slice cultures can be used to observe the behavior of dopaminergic neurons or other ventral midbrain neurons. Visualization of Caenorhabditis elegans Cuticle Structures using the Lipophilic Vital Dye, DiI Robbie D. Schultz1, 2, Tina L. Gumienny21Department of Molecular and Cellular Medicine, Texas A&M University System Health Science Center, 2Department of Molecular and Cellular Medicine, Texas A&M University System Health Science Center, College of Medicine We present a method to visualize cuticle in live C. elegans using the red fluorescent lipophilic dye DiI (1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate), which is commonly used in C. elegans to visualize environmentally exposed neurons. With this optimized protocol, alae and annular cuticular structures are stained by DiI and observed using compound microscopy. A Protocol for Detecting and Scavenging Gas-phase Free Radicals in Mainstream Cigarette Smoke Long-Xi Yu1, Boris G. Dzikovski2, Jack H. Freed3, 21CDCF-AOX Lab, 2National Biomedical Center for Advanced ESR Technology (ACERT), Department of Chemistry and Chemical Biology, Cornell University, 3ACERT Research, Center Department of Chemistry and Chemical Biology, Baker Laboratory, Cornell University Spin-trapping ESR spectroscopy was used to study the effect of plant antioxidants lycopene, pycnogenol and grape seed extract on scavenging gas-phase free radicals in cigarette smoke. How to Measure Cortical Folding from MR Images: A Step-by-step Tutorial to Compute Local Gyrification Index Marie Schaer1, Meritxell Bach Cuadra2, 3, Nick Schmansky4, Bruce Fischl4, Jean-Philippe Thiran2, Stephan Eliez11Department of Psychiatry, University of Geneva School of Medicine, 2Signal Processing Laboratory, École Polytechnique Fédérale de Lausanne, 3Department of Radiology, University Hospital Center and University of Lausanne, 4Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital Measuring gyrification (cortical folding) at any age represents a window into early brain development. Hence, we previously developed an algorithm to measure local gyrification at thousands of points over the hemisphere1. In this paper, we detail the computation of this local gyrification index. The Use of Thermal Infra-Red Imaging to Detect Delayed Onset Muscle Soreness Hani H. Al-Nakhli1, Jerrold S. Petrofsky1, 2, Michael S. Laymon2, Lee S. Berk11Loma Linda University, 2Azusa Pacific University The purpose of this investigation was to assess whether using an infra-red thermal camera is a valid tool for detecting and quantifying the muscle soreness after exercising.