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単離、特徴付けと二つの異なる方法を使用して永久歯に由来するヒト歯髄幹細胞の分化の比較
JoVE Journal
Medicine
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JoVE Journal Medicine
Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods
DOI:

14:52 min

November 24, 2012

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Chapters

  • 00:00Title
  • 01:18Introduction
  • 03:16Preparation of Enzyme Solution
  • 04:06Human Dental Pulp Isolation
  • 04:48Isolation of Dental Pulp Stem Cells by Enzymatic Dissociation of Dental Pulp Tissue
  • 06:28Outgrowth of Stem Cells from Tissue Explants
  • 07:27Immunophenotyping
  • 08:26Inducing Odontoblast Differentiation & Alizarin Red Staining Assay
  • 12:21Dental Pulp Stem Cells Culture and Differentiation Results
  • 14:14Conclusion

Summary

Automatic Translation

のいずれかを使用して人間の歯髄幹細胞(hDPSCs)の単離および特性を説明する方法<strongパルプ>酵素的解離(DPSC-ED)</strong>または<strong歯髄組織外植片からの幹細胞の>直接伸長(DPSC-OG)。その後に続く</strong><em> in vitroで</em象牙芽細胞へのhDPSCs両方のタイプの>比較分化。

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