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在体测量小鼠肺血管内皮表面层
JoVE Journal
Medicine
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JoVE Journal Medicine
In vivo Measurement of the Mouse Pulmonary Endothelial Surface Layer
DOI:

08:55 min

February 22, 2013

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Chapters

  • 00:05Title
  • 01:22Preparation of Surgical Tubing, Vascular Catheters and Chest Wall Window
  • 02:09Tracheostomy
  • 02:56Venous Catheterization
  • 03:43Intravital Mouse Lung Microscopy Surgery
  • 05:54Measurement of the Pulmonary Endothelial Surface Layer Thickness
  • 07:06Representative ESL Measurement Results
  • 08:31Conclusion

Summary

Automatic Translation

内皮细胞糖萼/内皮细胞表面层是理想的使用活体显微镜研究。活体显微镜技术上具有挑战性的运动器官如肺。我们展示了如何同时明场和荧光显微镜可用于估计内皮表面层的厚度,在可自由移动<em在体内</em>小鼠肺。

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