激光诱导的慢性高眼压表征视觉缺陷的小鼠模型
Summary
慢性高眼压诱导使用激光光凝小梁在小鼠眼睛。激光治疗后的几个月内升高的眼压（IOP）。减少实验动物的视力和对比敏感度使用optomotor测试监测。
Abstract
经常伴有高眼压（IOP），青光眼，失明的主要原因之一。我们试图建立高眼压症的小鼠模型来模拟人类高眼压性青光眼。这里激光照射施加到角膜缘photocoagulate水外流，诱导闭角。使用激光治疗之前和之后的反弹式眼压计的眼压的变化进行监测。是用于测量相应的变化在视觉能力一个optomotor的行为测试。代表性的结果显示从一个鼠标开发激光照射后持续高眼压。下降的视力，对比敏感度观察在高眼压鼠标。总之，我们的研究引入了一个有价值的模型系统研究青光眼的小鼠神经元变性的分子机制。
Protocol
程序 C57BL/6J小鼠（杰克逊实验室的Bar Harbor，ME）提出在西北大学的动物护理设施。按照协议，由西北大学机构动物护理和使用委员会，符合使用动物在神经科学的研究由美国国立卫生研究院的指引批准用于所有动物。 1。激光光凝从先前公布的5-7协议的程序修改光凝。 麻醉40-60日龄鼠标，腹腔内注射氯胺酮（100毫克/公斤…
Representative Results
中所描述的程序，目的是激光照明在角膜缘的区域photocoagulate水外流的小梁，诱导闭角型青光眼（ 图1）。大多数光刻眼睛表现出没有明显的物理伤害，色素脱落或感染，与以前的研究结果相一致6。当一小群小鼠（小于5％所有的光刻动物）展出，如的瘪眼球，严重的白内障，显着的色素支队，或出血严重损害的体征，我们立刻处死他们。约有30％的光刻眼睛轻微的角膜疤?…
Discussion
我们报告以上，持续的高眼压可诱发小鼠眼睛的激光照射。盐水注射模型18和静脉烧灼模型的11两者都需要广泛的显微技能相比，激光照射是相对简单和容易执行的。通常情况下，我们就可以进行激光照射4-6小鼠在2-3小时。实现持续的高眼压的关键步骤是前房压扁之前，激光和激光照射的参数。排水便于目标激光的小梁区和减少伤害到附近的睫状体和血管6的前房中的流体?…
Disclosures
The authors have nothing to disclose.
Acknowledgements
本文所载的工作已经得到了青光眼研究由美国健康援助基金会（XL），威廉＆玛丽格雷夫特别学术研究奖，以防止失明（XL），博士道格拉斯·约翰逊奖伊利诺伊防盲协会（HC）和美国国立卫生研究院：授予R01EY019034（XL）。
Materials
| Reagent | |||
| moxifloxacin | Alcon Labs, Inc. | NDC 0065-4013-03 | 0.5 %, Rx only |
| Proparacaine Hydrochloride | Bausch & Lomb | NDC 24208-730-06 | 0.5 %, Rx only |
| Ophthalmic Solution USP | Bausch & Lomb | NDC 24208-730-06 | .5 %, Rx only |
| ketamine | Butler Schein Animal Health | NDC 11695-0550-1 | 100 mg / kg |
| xylazine | LLOYD Inc. of Iowa | NADA 139-236 | 10 mg / kg |
| atropine sulfate solution | Alcon Labs, Inc. | NDC 61314-303-02 | 1 %, Rx only |
| Equipment | |||
| Slit Lamp, TOPCON | Visual Systems Inc | SL-3E | powered by PS-30A |
| OptoMotry 1.8.0 virtual | CerebralMechanics Inc. | ||
| opto-kinetic testing system | CerebralMechanics Inc. | ||
| Tonometer, TonoLab, for mice | Colonial Medical Supply | ||
| Heating pad | Sunbeam Products Inc | 722-810 | |
| Argon laser | Coherent Inc | Ultima 2000SE | |
| DECAPICONE Plastic cone holder | Braintree Sci Inc. | MDC-200 | for mouse |
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Cite This Article
Feng, L., Chen, H., Suyeoka, G., Liu, X. A Laser-induced Mouse Model of Chronic Ocular Hypertension to Characterize Visual Defects. J. Vis. Exp. (78), e50440, doi:10.3791/50440 (2013).