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Molecular Profiling of the Invasive Tumor Microenvironment in a 3-Dimensional Model of Colorectal Cancer Cells and Ex vivo Fibroblasts
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JoVE Journal Medicine
Molecular Profiling of the Invasive Tumor Microenvironment in a 3-Dimensional Model of Colorectal Cancer Cells and Ex vivo Fibroblasts

Molecular Profiling of the Invasive Tumor Microenvironment in a 3-Dimensional Model of Colorectal Cancer Cells and Ex vivo Fibroblasts

DOI:
10.3791/51475-v

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10:33 min

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April 29, 2014

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, , , , , , , ,
1Cancer Sciences Unit,University of Southampton School of Medicine, 2University Surgical Unit,University of Southampton School of Medicine, 3Bioinformatics Unit,London Research Institute, Cancer Research UK

Chapters

  • 00:05Title
  • 01:32Establishment of Primary Fibroblast Cultures from Colonic Explants
  • 03:18Organotypic Preparation
  • 06:52Organotypic Fixation and Laser Microdissection of the Invasive Margin
  • 08:47Results: MicroRNA Expression Profiling of Tissue from Laser-microdissected Organotypic CRC Models
  • 10:06Conclusion

Summary

Automatic Translation

Automatic Translation

Molecular profiling of laser microdissected cells and stroma from synthetic, tissue-engineered colorectal cancer models represents a novel and manipulatable approach to characterize and dissect the distinctive biology at the interface between tumor cells at the invasive front and cancer associated stromal cells.  

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Molecular ProfilingInvasive Tumor Microenvironment3D Colorectal Cancer ModelEx Vivo FibroblastsTumor InvasionMetastatic CascadeCancer-Associated StromaOrganotypic Co-CultureLaser MicrodissectionGenomic/Transcriptomic/Epigenetic ProfilingBiomarkersDrug Development

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