JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Medicine
/
在结直肠癌细胞的三维模型的入侵肿瘤微环境的分子剖析和<em>体外</em>成纤维细胞
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Medicine
Molecular Profiling of the Invasive Tumor Microenvironment in a 3-Dimensional Model of Colorectal Cancer Cells and
Ex vivo
Fibroblasts
Please note that all translations are automatically generated.
Click here for the English version.
在结直肠癌细胞的三维模型的入侵肿瘤微环境的分子剖析和<em>体外</em>成纤维细胞
DOI:
10.3791/51475-v
•
10:33 min
•
April 29, 2014
•
Marc D. Bullock
2
,
Max Mellone
,
Karen M. Pickard
,
Abdulkadir Emre Sayan
,
Richard Mitter
,
John N. Primrose
,
Graham K. Packham
,
Gareth Thomas
,
Alexander H. Mirnezami
2
1
Cancer Sciences Unit
,
University of Southampton School of Medicine
,
2
University Surgical Unit
,
University of Southampton School of Medicine
,
3
Bioinformatics Unit
,
London Research Institute, Cancer Research UK
Chapters
00:05
Title
01:32
Establishment of Primary Fibroblast Cultures from Colonic Explants
03:18
Organotypic Preparation
06:52
Organotypic Fixation and Laser Microdissection of the Invasive Margin
08:47
Results: MicroRNA Expression Profiling of Tissue from Laser-microdissected Organotypic CRC Models
10:06
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
激光显微切割细胞和基质由合成的,组织工程肠癌模型的分子分析代表了一种新的和可操作的方法在肿瘤细胞之间的界面来表征和解剖的独特生物学在浸润前沿和癌症相关的基质细胞。
Tags
Molecular Profiling
Invasive Tumor Microenvironment
3D Colorectal Cancer Model
Ex Vivo Fibroblasts
Tumor Invasion
Metastatic Cascade
Cancer-Associated Stroma
Organotypic Co-Culture
Laser Microdissection
Genomic/Transcriptomic/Epigenetic Profiling
Biomarkers
Drug Development
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
check_url/51475?article_type=v
3维建模和成像集体细胞的侵袭
check_url/51475?article_type=v
对黑色素瘤修饰符一个斑马鱼原地肿瘤模型的筛选
check_url/51475?article_type=v
实时成像在乳腺癌小鼠模型中的肿瘤微环境对药物的反应
check_url/51475?article_type=v
一种新型的高分辨率<em>在体内</em>成像技术研究肿瘤生长及治疗颅内结构的动态响应
check_url/51475?article_type=v
乳腺癌的一种新的转基因小鼠模型:转移性乳腺癌通过瞄准导管上皮与腺病毒Cre重组酶的启动
check_url/51475?article_type=v
一个<em>体外</em>模型在乳腺癌研究激素的作用
check_url/51475?article_type=v
三维(3D)肿瘤球体侵袭试验
check_url/51475?article_type=v
研究胰腺癌干细胞特性开发新的治疗策略
check_url/51475?article_type=v
从小鼠癌症模型的循环肿瘤细胞的分离和繁殖
check_url/51475?article_type=v
脑肿瘤/器官切片共培养系统研究肿瘤微环境和靶向药物治疗
Read Article