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在结直肠癌细胞的三维模型的入侵肿瘤微环境的分子剖析和<em>体外</em>成纤维细胞

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Medicine

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JoVE Journal Medicine

Molecular Profiling of the Invasive Tumor Microenvironment in a 3-Dimensional Model of Colorectal Cancer Cells and Ex vivo Fibroblasts

Please note that all translations are automatically generated. Click here for the English version.

在结直肠癌细胞的三维模型的入侵肿瘤微环境的分子剖析和<em>体外</em>成纤维细胞

DOI:

10.3791/51475-v

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10:33 min

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April 29, 2014

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Marc D. Bullock², Max Mellone, Karen M. Pickard, Abdulkadir Emre Sayan, Richard Mitter, John N. Primrose, Graham K. Packham, Gareth Thomas, Alexander H. Mirnezami²

¹Cancer Sciences Unit,University of Southampton School of Medicine, ²University Surgical Unit,University of Southampton School of Medicine, ³Bioinformatics Unit,London Research Institute, Cancer Research UK

Chapters

00:05Title
01:32Establishment of Primary Fibroblast Cultures from Colonic Explants
03:18Organotypic Preparation
06:52Organotypic Fixation and Laser Microdissection of the Invasive Margin
08:47Results: MicroRNA Expression Profiling of Tissue from Laser-microdissected Organotypic CRC Models
10:06Conclusion

Summary

Automatic Translation

激光显微切割细胞和基质由合成的，组织工程肠癌模型的分子分析代表了一种新的和可操作的方法在肿瘤细胞之间的界面来表征和解剖的独特生物学在浸润前沿和癌症相关的基质细胞。

Tags

Molecular Profiling Invasive Tumor Microenvironment 3D Colorectal Cancer Model Ex Vivo Fibroblasts Tumor Invasion Metastatic Cascade Cancer-Associated Stroma Organotypic Co-Culture Laser Microdissection Genomic/Transcriptomic/Epigenetic Profiling Biomarkers Drug Development

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