<em>In Vivo</em> Microinjection and Electroporation of Mouse Testis

Marten Michaelis; Alexander Sobczak; Joachim M. Weitzel

doi:10.3791/51802

Journal

/

Biology

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在小鼠睾丸活体显微注射和电穿孔

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In Vivo Microinjection and Electroporation of Mouse Testis

Please note that all translations are automatically generated. Click here for the English version.

在小鼠睾丸活体显微注射和电穿孔

DOI:

10.3791/51802-v

•

08:39 min

•

August 23, 2014

•

Marten Michaelis, Alexander Sobczak, Joachim M. Weitzel

¹Institute of Reproductive Biology,Leibniz Institute for Farm Animal Biology (FBN)

Chapters

00:05Title
02:11Anesthesia and Surgery
03:41Microinjection and DNA Application
04:51Electroporation of Testis
05:32Wound Closure and Post-surgery
06:41Results: In Vivo Microinjection and Electroporation of Mouse Testis
07:28Conclusion

Summary

Automatic Translation

本文介绍了显微注射和小鼠睾丸中电在体内的睾丸小鼠细胞转染技术，研究精子的独特工艺。所提出的方案涉及以下步骤：玻璃毛细管制备，经由输出管显微注射，并通过电穿孔转染。

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