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Journal
/
Biology
/
在
小鼠睾丸
活体
显微注射和电穿孔
JoVE Journal
Biology
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JoVE Journal
Biology
In Vivo
Microinjection and Electroporation of Mouse Testis
Please note that all translations are automatically generated.
Click here for the English version.
在
小鼠睾丸
活体
显微注射和电穿孔
DOI:
10.3791/51802-v
•
08:39 min
•
August 23, 2014
•
Marten Michaelis
,
Alexander Sobczak
,
Joachim M. Weitzel
1
Institute of Reproductive Biology
,
Leibniz Institute for Farm Animal Biology (FBN)
Chapters
00:05
Title
02:11
Anesthesia and Surgery
03:41
Microinjection and DNA Application
04:51
Electroporation of Testis
05:32
Wound Closure and Post-surgery
06:41
Results: In Vivo Microinjection and Electroporation of Mouse Testis
07:28
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文介绍了显微注射和小鼠睾丸中电
在体内
的睾丸小鼠细胞转染技术,研究精子的独特工艺。所提出的方案涉及以下步骤:玻璃毛细管制备,经由输出管显微注射,并通过电穿孔转染。
Tags
In Vivo Microinjection
Electroporation
Mouse Testis
Transfection
Spermatogenesis
Reporter Genes
Gene Transfer
Gene Function Analysis
DNA Constructs
RNA Constructs
Gene Regulation
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