胰腺管输液：药物和病毒输送的有效和选择性方法
Summary
胰腺导管输液是一种重要的技术，可用于谱系追踪、基因导入和细胞系特定靶向。这里介绍了用于药物和病毒输送到胰腺细胞的胰腺导管输液技术。
Abstract
胰腺是一种双功能器官，具有内分泌和外分泌成分。许多病理学可能折磨胰腺，包括糖尿病、胰腺炎和胰腺癌。这三种疾病都标志着活跃的研究领域，不仅是为了开发即时疗法，而且是为了更好地了解它们的病理生理学。很少有工具可以进一步研究这些领域。胰腺导管输液是一种重要的技术，可用于谱系追踪、基因导入和细胞系特定靶向。该技术需要对十二指肠和安普拉的第二部分进行复杂的解剖，然后是胆管的闭塞和胰腺导管的闭合。虽然这项技术在技术上起初具有挑战性，但应用却不尽人格。各组之间程序细节的模糊性突出了制定标准协议的必要性。本工作描述了在胰腺内的绿色荧光蛋白 （GFP） 的表达后，胰腺内的病毒载体注入病毒载体，表达 GFP 与虚假手术。输液和因此表达是特定于胰腺，没有表达存在于任何其他组织类型。
Introduction
胰腺是一种双功能器官，具有内分泌和外分泌成分。许多病理学可能折磨胰腺，包括糖尿病、胰腺炎和胰腺癌。这三种疾病都标志着活跃的研究领域，不仅是为了开发即时疗法，而且是为了更好地了解它们的病理生理学。
有针对性的治疗交付方法将是有益的。我们开发了胰腺导管输液技术，这是药物和病毒输送到胰腺细胞的有效选择性方法。在这个模型中，胰腺导管有选择地被封装，普通胆管被遮盖，允许专门输送到胰腺。
该技术可以使特定细胞类型的血统追踪进一步阐明对胰腺发育和病理学重要的发育路径2，3。病毒载体中的不同促进者允许细胞系的特定靶向，并允许将基因引入这些细胞系4、5。这项工作展示了在胰腺内的绿色荧光蛋白（GFP）的表达后，胰腺内的胰腺管道注入病毒载体表达GFP与虚假手术。
Protocol
Representative Results
Discussion
本研究详细描述了胰腺导管输注背后的方法，这是一种有效的小鼠模型，用于将基因和其他分子具体而有效地输送到胰腺。各组之间程序细节的模糊性突出了标准化协议8、9、10的必要性。
有几个关键步骤的程序，从选择年轻，健康的老鼠开始。十二指肠穿孔，无论多么小和可控，是鼠标中的创伤性事件，…
Disclosures
The authors have nothing to disclose.
Acknowledgements
作者没有承认。浙江省卫生与计划生育委员会基金会（20146242赠款）和温州市科技局基金会（赠款Y20140248）提供了资助。
Materials
| Mice (25-30g, 8-12 weeks old) | Jackson Laboratory | ||
| Ketofen | Henry Schein Inc. | 5487 | |
| Ethanol (70%) | Sigma-Aldrich | 34923 | |
| Sterile Saline Solution | Baxter Healthcare Corp. | 2B-13-00 | |
| Protective Equipment | |||
| Hair Removal Product | Nair or trimmer | ||
| Gauze Pads 2in x 2in | Fisher Healthcare | 22-362-178 | |
| Needles (30.5G and 25G) | Becton Dicksinson and Co. | 305106 and 305122 | |
| Syringe for Ketofen and Saline | Becton Dicksinson and Co. | 309657 | |
| Syringe for Pump | Henke Sass Wolf | 4010.200V0 | |
| Infusion Pump | Pump Systems Inc. | NE-1000 | |
| Infusion Catheter | World Precision Instruments | CMF31G | |
| Curved Bulldog Clamps (2) | Roboz | RS-7439 | |
| Arruga Forceps | Roboz | RS-5163 | |
| Adson Forcep | Roboz | RS-5234 | |
| Needle Holder | Roboz | RS-7882 | |
| Scissor | Roboz | RS-5982 | |
| Cotton Tip Applicators | Physician Sales and Services | 22-9988 | |
| Dissecting Microscope | |||
| Suture | Owens and Minor | SXMD1B402 |
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