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用于固相合成含有2'-<em> 0</em通过圆二色性修饰和表征
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Chemistry
Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2′-
O
-thiophenylmethyl Modification and Characterization via Circular Dichroism
Please note that all translations are automatically generated.
Click here for the English version.
用于固相合成含有2'-<em> 0</em通过圆二色性修饰和表征
DOI:
10.3791/56189-v
•
11:37 min
•
July 28, 2017
•
Andrew J. Francis
,
Marino J. E. Resendiz
1
Department of Chemistry
,
University of Colorado Denver
Chapters
00:05
Title
01:07
Solid-phase Synthesis of RNA Oligonucleotides
03:25
Deprotection and Purification of RNA Oligonucleotides
07:12
Characterization via MALDI-TOF and RNA Structure Analysis vi CD
09:24
Results: Characterization of RNA Oligomers Containing 2′-
O
thiophenylmethyl Modification
10:25
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文提供了在C2'-
O
位上修饰的RNA的十二聚体的固相合成,纯化和表征的详细程序。 UV-vis和圆二色性光度分析用于量化和表征结构方面,
即
单链或双链。
Tags
Solid-phase Synthesis
Oligomers
RNA
2′-O-thiophenylmethyl Modification
Circular Dichroism
Phosphoramidite Chemistry
Oligonucleotides
Therapeutic Applications
Peptide Synthesizer
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