Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-<em>O</em>-thiophenylmethyl Modification and Characterization via Circular Dichroism

Andrew J. Francis; Marino J. E. Resendiz

doi:10.3791/56189

Journal

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Chemistry

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用于固相合成含有2'-<em> 0</em通过圆二色性修饰和表征

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JoVE Journal Chemistry

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2′-O-thiophenylmethyl Modification and Characterization via Circular Dichroism

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用于固相合成含有2'-<em> 0</em通过圆二色性修饰和表征

DOI:

10.3791/56189-v

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11:37 min

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July 28, 2017

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Andrew J. Francis, Marino J. E. Resendiz

¹Department of Chemistry,University of Colorado Denver

Chapters

00:05Title
01:07Solid-phase Synthesis of RNA Oligonucleotides
03:25Deprotection and Purification of RNA Oligonucleotides
07:12Characterization via MALDI-TOF and RNA Structure Analysis vi CD
09:24Results: Characterization of RNA Oligomers Containing 2′-Othiophenylmethyl Modification
10:25Conclusion

Summary

Automatic Translation

本文提供了在C2'- O位上修饰的RNA的十二聚体的固相合成，纯化和表征的详细程序。 UV-vis和圆二色性光度分析用于量化和表征结构方面，即单链或双链。

用于固相合成含有2'-<em> 0</em通过圆二色性修饰和表征

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