Journal
/
/
Förster Time-resolved omogeneo risonanza energia basati su trasferimento test per la determinazione della secrezione dell'insulina
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
Homogeneous Time-resolved Förster Resonance Energy Transfer-based Assay for Detection of Insulin Secretion
DOI:

07:30 min

May 10, 2018

, ,

Chapters

  • 00:05Title
  • 01:22INS-1E Cells: Maintenance and Plating
  • 03:04Insulin Secretion Assay
  • 04:12HTRF To Measure Insulin Secretion and Data Analysis
  • 05:17Results: Use of HTRF Assay for the Rapid Detection of Insulin Secretion from the Cells
  • 06:43Conclusion

Summary

Automatic Translation

Qui, presentiamo omogeneo tempo risolto FRET (HTRF) come un metodo efficace per la rilevazione rapida di insulina secernuta dalle cellule.

Related Videos

Read Article