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Journal
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Biochemistry
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体外
检测 tRNA-Isopentenyl 转移酶活性的实验研究
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
An
In Vitro
Assay to Detect tRNA-Isopentenyl Transferase Activity
Please note that all translations are automatically generated.
Click here for the English version.
体外
检测 tRNA-Isopentenyl 转移酶活性的实验研究
DOI:
10.3791/58100-v
•
07:46 min
•
October 08, 2018
•
Antonio E. Chambers*
1
,
Adam E. Richardson*
1
,
David F. Read
,
Thomas J. Waller
,
Douglas A. Bernstein
,
Philip J. Smaldino
1
Department of Biology
,
Ball State University
,
2
Department of Genome Sciences
,
University of Washington
,
3
Department of Molecular, Cellular, and Developmental Biology
,
University of Michigan
Chapters
00:04
Title
00:39
Preparing a 20% Polyacrylamide Denaturing Gel
02:17
RNA Isopentenylation Assay
05:14
Results: Detection of Isopentenylation by Mod5
06:42
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里, 我们描述了一个协议的生化特性的酵母 rna 修饰酶, Mod5, 并讨论如何将该协议适用于其他 RNA 修饰酶。
Tags
TRNA-Isopentenyl Transferase
In Vitro Assay
RNA Modification
Biochemical Characterization
Recombinant Enzymes
RNA Modifying Enzymes
Acrylamide Gel
Urea Gel
TBE Buffer
RNA Isopentenylation Assay
DMAPP
Mod5
2-mercaptoethanol
RNA Precipitation
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