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Journal
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Immunology and Infection
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マウスと次世代シーケンサーによるひと抗体のレパートリーの同定
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing
Please note that all translations are automatically generated.
Click here for the English version.
マウスと次世代シーケンサーによるひと抗体のレパートリーの同定
DOI:
10.3791/58804-v
•
08:51 min
•
March 15, 2019
•
Lin Sun
,
Naoko Kono
,
Hiroyuki Toh
,
Hanbing Xue
,
Kaori Sano
5
,
Tadaki Suzuki
,
Akira Ainai
,
Yasuko Orba
,
Junya Yamagishi
8
,
Hideki Hasegawa
5
,
Yoshimasa Takahashi
,
Shigeyuki Itamura
,
Kazuo Ohnishi
10
1
Graduate School of Life and Environmental Sciences
,
University of Tsukuba
,
2
Center for Influenza Virus Research
,
National Institute of Infectious Diseases
,
3
School of Science and Technology
,
Kwansei Gakuin University
,
4
Department of Pathology
,
National Institute of Infectious Diseases
,
5
Division of Infectious Diseases Pathology, Department of Global Infectious Diseases
,
Tohoku University Graduate School of Medicine
,
6
Division of Molecular Pathobiology, Research Center for Zoonosis Control
,
Hokkaido University
,
7
Division of Collaboration and Education, Research Center for Zoonosis Control
,
Hokkaido University
,
8
Global Station for Zoonosis Control, GI-CoRE
,
Hokkaido University
,
9
Department of Immunology
,
National Institute of Infectious Diseases
,
10
Faculty of Life and Environmental Sciences
,
University of Tsukuba
Chapters
00:04
Title
01:23
Nucleic Acid Isolation from Immune Cells and Tissues
03:04
cDNA Synthesis and PCR Amplification
04:58
NGS Sequencing of Libraries
05:33
Results: Identification of Mouse and Human Antibody Repertoires
08:11
Conclusion
Summary
Automatic Translation
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Automatic Translation
ここでは、分析と構造の可視化と全体抗体レパートリーの憲法のためのプロトコルについて述べる。次世代シーケンシングによる抗体 RNA の広大な配列の取得が含まれます。
Tags
Antibody Repertoire
Next-generation Sequencing
B Cell Repertoire
Immune Response
Vaccine Efficacy
Immunoglobulin Amplification
RNA Extraction
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