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通过Sialoglycan的代谢标记识别主要神经干细胞和祖细胞的细胞表面标记
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Neuroscience
Identifying Cell Surface Markers of Primary Neural Stem and Progenitor Cells by Metabolic Labeling of Sialoglycan
Please note that all translations are automatically generated.
Click here for the English version.
通过Sialoglycan的代谢标记识别主要神经干细胞和祖细胞的细胞表面标记
DOI:
10.3791/58945-v
•
11:39 min
•
September 07, 2019
•
Qing-Ran Bai*
1
,
Lu Dong*
2
,
Qin Shen
1
Brain and Spinal Cord Innovative Research Center of Tongji Hospital, School of Life Sciences and Technology
,
Tongji University and Frontier Science Research Center for Stem Cells of Ministry of Education
,
2
College of Chemistry and Molecular Engineering, Peking-Tsinghua Center for Life Sciences, Beijing National Laboratory for Molecular Sciences, Synthetic and Functional Biomolecules Center, and Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education
,
Peking University
Chapters
00:05
Title
01:16
Preparation of Mouse Endothelial Culture in Permeable Support Inserts
01:55
Set-up of Neural-endothelial Co-culture and Ac
4
ManNAz Labeling System
03:25
Immunofluorescent Staining of Sialoglycoproteins in Expanded Primary NSPCs and Differentiated Neurons
05:25
Purification of Sialoglycoproteins from Expanded Primary NSPCs and Differentiated Neurons
08:45
Results: Identifying Cell Surface Markers by Metabolic Labeling of Sialoglycan
10:47
Conclusion
Summary
Automatic Translation
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Automatic Translation
这里介绍的是一个协议,结合了
体外
神经内皮共培养系统和代谢结合的异形理念功能组,以扩大主神经干和祖细胞,并标记其表面用于细胞表面标记的成像或质谱分析的紫锥蛋白。
Tags
Neural Stem Cells
Neural Progenitor Cells
Cell Surface Markers
Metabolic Labeling
Sialoglycan
Endothelial Cell Co-culture
Ac4ManAz
MAPK-ERK Pathway
Primary Cortical Cells
Cell Purification
Regenerative Medicine
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