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Isolamento de neutrófilos de sangue periférico humano para interrogar o Caminho Associado lRRK2 Kinase de Parkinson avaliando rab10 fosforilação
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1MRC Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences,University of Dundee, 2The Michael J. Fox Foundation for Parkinson’s Research, 3Department of Medical and Molecular Genetics,Indiana University School of Medicine, 4Morton and Gloria Shulman Movement Disorders Centre and the Edmond J. Safra Program in Parkinson’s Disease, Toronto Western Hospital,University of Toronto, 5Division of Cell Signalling and Immunology, School of Life Sciences,University of Dundee, 6Division of Molecular and Clinical Medicine, School of Medicine, Ninewells Hospital and Medical School,University of Dundee
Chapters
- 00:01Introduction
- 00:48Neutrophil Isolation from Human Peripheral Blood
- 07:18LRRK2 Kinase Inhibitor Treatment
- 10:33Results
- 12:14Conclusion
Mutações no rico gene de leucina repetição quinase 2 (LRRK2) causam doença hereditária de Parkinson. Desenvolvemos um método fácil e robusto para avaliar a fosforilação controlada por LRRK2 de Rab10 em neutrófilos de sangue periféricos humanos. Isso pode ajudar a identificar indivíduos com aumento da atividade da via de quinase LRRK2.
